Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry
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DOI：
10.1038/nm1371
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发表时间：
2006-08-01
期刊：
影响因子：
82.9
通讯作者：
Roederer, Mario
中科院分区：
文献类型：
--
作者：
Chattopadhyay, Pratip K.;Price, David A.;Roederer, Mario
免疫反应产生于表达多种细胞表面蛋白独特组合的多种细胞。然而，由于可用的探针和仪器的限制，详细的表征受到阻碍。在这里，我们利用半导体纳米晶体（量子点）独特的光谱特性来扩展多色流式细胞术的能力，以解决17个荧光发射。我们通过详细分析多种抗原特异性t细胞群的表型，揭示了复杂表型模式中的变化，从而证明了这种能力的必要性，否则这些变化将仍然是模糊的。例如，对一种病原体的不同表位特异的T细胞，甚至对相同表位特异的T细胞，可能具有明显不同的表型。我们描述的技术，包括八个量子点与传统荧光团的检测，应该扩大流式细胞术的视野，以及我们表征适应性和先天细胞免疫反应复杂性的能力。
Immune responses arise from a wide variety of cells expressing unique combinations of multiple cell-surface proteins. Detailed characterization is hampered, however, by limitations in available probes and instrumentation. Here, we use the unique spectral properties of semiconductor nanocrystals (quantum dots) to extend the capabilities of polychromatic flow cytometry to resolve 17 fluorescence emissions. We show the need for this power by analyzing, in detail, the phenotype of multiple antigen-specific T-cell populations, revealing variations within complex phenotypic patterns that would otherwise remain obscure. For example, T cells specific for distinct epitopes from one pathogen, and even those specific for the same epitope, can have markedly different phenotypes. The technology we describe, encompassing the detection of eight quantum dots in conjunction with conventional fluorophores, should expand the horizons of flow cytometry, as well as our ability to characterize the intricacies of both adaptive and innate cellular immune responses.