Rhamnolipids Mediate an Interspecies Biofilm Dispersal Signaling Pathway
Rhamnolipids Mediate an Interspecies Biofilm Dispersal Signaling Pathway
复制标题
DOI：
10.1021/acschembio.6b00750
复制
发表时间：
2016-11-01
影响因子：
4
通讯作者：
Hochbaum, Allon I.
中科院分区：
文献类型：
--
作者：
Bhattacharjee, Arunima;Nusca, Tyler D.;Hochbaum, Allon I.
细菌生物膜在自然和人为环境中都是有问题的，它们对其组成细胞具有保护作用，使它们很难用常规抗生素治疗。因此，抗生物被膜策略代表了治疗生物被膜感染的一个有前途的研究方向。自然自我扩散和物种间扩散信号通路提供了对细胞细胞通信机制、混合群落中的物种动态以及感染治疗的潜在靶点的洞察。在这里，我们描述了一种新的铜绿假单胞菌和大肠杆菌之间的跨物种扩散信号通路。铜绿假单胞菌培养上清液中的化合物使大肠杆菌生物膜分散。铜绿假单胞菌LAS和RHL群体感应系统的两个组分，N-(3-氧代十二酰)高丝氨酸内酯(3oxoC12HSL)和鼠李糖脂，被发现协同作用分散大肠杆菌生物膜。我们的结果表明，鼠李糖脂不影响生长、生物膜的发育或在大肠杆菌中的扩散，而是通过诱导大肠杆菌膜的选择性通透性来补充3oxoC12HSL信号。靶细胞通透性的增加与鼠李糖脂介导的从大肠杆菌膜上去除脂多糖是一致的，并且似乎选择性地增加了亲脂性酰基高丝氨酸内酯的通透性。这项工作表明鼠李糖脂在铜绿假单胞菌种间信号转导中起着关键作用。鼠李糖脂和其他生物表面活性剂在其他种内和种间化学信号通路中可能具有类似的作用。
Bacterial biofilms are problematic in natural and anthropogenic environments, and they confer protective properties on their constituent cells, making them difficult to treat with conventional antibiotics. Antibiofilm strategies, therefore, represent a promising direction of research for treating biofilm infections. Natural autodispersal and inter species dispersal signaling pathways provide insight into cell cell communication mechanisms, species dynamics in mixed communities, and potential targets for infection therapies. Here, we describe a novel interspecies dispersal signaling pathway between Pseudomonas aeruginosa and Escherichia coli. E. coli biofilms disperse in response to compounds in P. aeruginosa culture supernatant. Two components of the P. aeruginosa Las and Rhl quorum sensing systems, N-(3-oxo-dodecanoyl) homoserine lactone (3oxoC12HSL) and rhamnolipids, are found to act cooperatively to disperse E. coli biofilms. Our results indicate that rhamnolipids do not affect growth, biofilm development, or dispersal in E. coli but instead complement 3oxoC12HSL signaling by inducing selective permeability of the E. coli membrane. The increased target cell permeability is consistent with rhamnolipid-mediated removal of lipopolysaccharide from E. coli membranes and appears to selectively increase the permeability of lipophilic acyl homoserine lactones. This work suggests that rhamnolipids play a critical role in P. aeruginosa E. coli interspecies signaling. Rhamnolipids and other biosurfactants may have similar effects in other intra- and interspecies chemical signaling pathways.