Genetic diversity of enterovirus 71 associated with hand, foot and mouth disease epidemics in Japan from 1983 to 2003
Genetic diversity of enterovirus 71 associated with hand, foot and mouth disease epidemics in Japan from 1983 to 2003
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DOI：
10.1097/01.inf.0000227959.89339.c3
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发表时间：
2006-08-01
影响因子：
3.6
通讯作者：
Suzuki, Hitoshi
中科院分区：
文献类型：
--
作者：
Hosoya, Mitsuaki;Kawasaki, Yukihiko;Suzuki, Hitoshi
背景：肠病毒71型（EV71）是手足口病（HFMD）的主要病原之一。监测数据表明，EV71病毒感染遵循流行传播模式，引起大规模暴发，然后沉寂数年。方法：对1983 ~ 2003年日本福岛地区手足口病患者分离的121株EV71型病毒进行遗传多样性分析，并与国际上分离的164株EV71型病毒进行VP4序列系统发育分析。结果：福岛县1984年、1987年、1990年、1993年、1997年、2000年和2003年分别有ev71型相关手足口病暴发。在福岛分离的EV71病毒株的系统发育重建显示出8个遗传上不同的聚类，包括6个亚群（先前命名为B-1、B-2和3、B-4、C-1、C-2和C-3）和2个亚群（新命名为B-5和C-4）。另外2个不明显的簇属于基因组C，命名为G-UI和C- u2。在这些亚群中，B-1、C-U1、C-U2、C-2、134以及C-4和B-5主要与分别发生在1984年、1987年和1990年、1993年、1997年、2000年和2003年的流行病有关。来自福岛每次暴发的EV71毒株与几乎同一时期在日本其他地区和其他国家分离的毒株形成了一个单一的集群。结论：我们的研究结果表明，EV71病毒的反复暴发可能是新引入的遗传变异EV71病毒在世界范围内传播的结果。
Background: Enterovirus 71 (EV71) is one of the major etiologic agents of hand, foot and mouth disease (HFMD). The surveillance data indicate that EV71 infection follows an epidemic mode of transmission, causing large outbreaks and then becoming quiescent for a few years.Methods: We investigated the genetic diversity of a total of 121 EV71 strains isolated from patients with HFMD in Fukushima, Japan, from 1983 to 2003 and compared their genetic relation with the 164 EV71 strains isolated in the world using phylogenetic analysis based on the VP4 sequence.Results: We observed EV71-related HFMD outbreaks in Fukushima in 1984, 1987, 1990, 1993, 1997, 2000 and 2003. Phylogenetic reconstruction of EV71 strains isolated in Fukushima demonstrated 8 genetically distinct clusters, including 6 subgroups previously designated as B-1, B-2 and 3, B-4, C-1, C-2, and C-3 and 2 subgroups newly designated as B-5 and C-4. Additional 2 indistinct clusters belonged to genogroup C and were named G-UI and C-U2. Of those subgroups, B-1, C-U1, C-U2, C-2, 134, and C-4 and B-5 dominantly related to epidemics that occurred in the years 1984, 1987 and 1990, 1993, 1997, 2000 and 2003, respectively. EV71 strains derived from each outbreak in Fukushima formed a single cluster with those isolated during almost the same time period in other area of Japan and in other countries.Conclusions: Our results suggested that the repeated EV71 outbreaks might be the result of the worldwide transmission of the newly introduced genetically divergent EV71 strains.