Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins.
Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins.
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枯草芽孢杆菌 ANSB060 对黄曲霉毒素污染鸭饲料中生长性能、抗氧化功能和黄曲霉毒素残留的改善作用
DOI：
10.3390/toxins9010001
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发表时间：
2016-12-22
期刊：
影响因子：
4.2
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Zhang L;Ma Q;Ma S;Zhang J;Jia R;Ji C;Zhao L
枯草芽孢杆菌ANSB 060是从鱼内脏中分离的一株对饲料和饲料配料中黄曲霉毒素具有很好的解毒作用的菌株。本研究的目的是探讨B的作用。枯草杆菌ANSB 060对饲喂黄曲霉毒素污染玉米的鸭的生长性能、机体抗氧化功能和黄曲霉毒素残留的影响。选用1500只体重相近的18日龄樱桃谷公鸭，随机分为5个处理，每个处理6个重复，每个重复50只。试验设计包括5个处理，分别为C 0（基础日粮中含60%正常玉米）、M0（基础日粮中含60%黄曲霉毒素污染玉米）、M500、M1000和M2000（M0 + 500、1000和2000 g/t黄曲霉毒素降解制剂主要由B组成）。subtilis ANSB 060）。结果表明，与C组相比，添加22.44 ± 2.46 μg/kg黄曲霉毒素B1（M0）组鸭的日增重和血清总超氧化物歧化酶（T-SOD）活性呈下降趋势，肝脏T-SOD和谷胱甘肽过氧化物酶（GSH-Px）活性沿着黄曲霉毒素B1和黄曲霉毒素M1的出现而显著下降。补充B。枯草杆菌ANSB 060添加到黄曲霉毒素污染的日粮中，提高了鸭的平均日增重（p > 0.05），显著提高了抗氧化酶活性，降低了黄曲霉毒素在鸭肝中的积累。因此，枯草芽孢杆菌ANSB 060对鸭黄曲霉毒素病有一定的改善作用，是一种很有前景的饲料添加剂。
Bacillus subtilis ANSB060 isolated from fish gut is very effective in detoxifying aflatoxins in feed and feed ingredients. The purpose of this research was to investigate the effects of B. subtilis ANSB060 on growth performance, body antioxidant functions, and aflatoxin residues in ducks fed moldy maize naturally contaminated with aflatoxins. A total of 1500 18-d-old male Cherry Valley ducks with similar body weight were randomly assigned to five treatments with six replicates of 50 ducks per repeat. The experiment design consisted of five dietary treatments labeled as C0 (basal diet containing 60% normal maize), M0 (basal diet containing 60% moldy maize contaminated with aflatoxins substituted for normal maize), M500, M1000, and M2000 (M0 +500, 1000 or 2000 g/t aflatoxin biodegradation preparation mainly consisted of B. subtilis ANSB060). The results showed that ducks fed 22.44 ± 2.46 μg/kg of AFB1 (M0) exhibited a decreasing tendency in average daily gain (ADG) and total superoxide dismutase (T-SOD) activity in serum, and T-SOD and glutathione peroxidase (GSH-Px) activities in the liver significantly decreased along with the appearance of AFB1 and AFM1 compared with those in Group C0. The supplementation of B. subtilis ANSB060 into aflatoxin-contaminated diets increased the ADG of ducks (p > 0.05), significantly improved antioxidant enzyme activities, and reduced aflatoxin accumulation in duck liver. In conclusion, Bacillus subtilis ANSB060 in diets showed an ameliorating effect to duck aflatoxicosis and may be a promising feed additive.