Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid
Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid
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DOI：
10.1016/j.imbio.2010.03.008
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发表时间：
2011-01-01
期刊：
影响因子：
2.8
通讯作者：
Hermann, Corinna
中科院分区：
文献类型：
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作者：
Dehus, Oliver;Pfitzenmaier, Markus;Hermann, Corinna
研究了单核细胞增生李斯特菌中脂磷壁酸（LTA）的表达，利用NMR和MS技术发现了LTA的两种不同结构变体（LTA 1和LTA 2）。虽然两种LTA均由通过二糖与二酰基甘油部分结合的聚甘油磷酸骨架（长度不同）组成，但一种LTA类型（LTA 2）具有通过磷酸二酯与二糖连接的第二个二酰基甘油部分。如在体外检查的，与LTA 1相反，LTA 2不能激活补体的L-纤维胶凝蛋白依赖性途径。最有趣的是，生长温度对LTA 1和LTA 2在细胞壁中的表达水平具有强烈的影响：虽然LTA 1的量相当，但当李斯特菌在室温下生长时LTA 2的表达较低（LTA 1与LTA 2的比例为1：0.06），但当李斯特菌在37 ℃下培养时LTA 2的表达增加（LTA 1与LTA 2的比例为1：0.68）。所观察到的LTA表达的转变，可能伴随着从有害物质到毒性实体的转换，表明对宿主细胞内不同结构要求的重要适应。(C)2010年Elsevier GmbH。All rights reserved.
Investigating the expression of lipoteichoic acid (LTA) from Listeria monocytogenes, we found two distinct structural variants of LTA (LTA1 and LTA2) using NMR and MS technology. While both LTA consisted of a poly-glycerophosphate backbone (differing in length) bound via a disaccharide to a diacyl-glycerol moiety, one LTA type (LTA2) possessed a second diacyl-glycerol moiety linked to the disaccharide via a phosphodiester. As examined in vitro, LTA2 in contrast to LTA1 failed to activate the L-ficolin dependent pathway of complement. Most interestingly, growth temperature had a strong influence on the expression levels of LTA1 and LTA2 in the cell wall: while the amount of LTA1 was comparable, the expression of LTA2 was low when Listeria had grown at room temperature (ratio of LTA1 to LTA2 was 1:0.06), but increased when Listeria had been cultivated at 37 degrees C (ratio of LTA1 to LTA2 was 1:0.68). The observed shift in LTA expression, probably accompanying the switch from the saprophytic to the virulent entity, indicates an important adaptation to the different structural requirements inside the host cells. (C) 2010 Elsevier GmbH. All rights reserved.