Photothermally induced local dissociation of collagens for harvesting of cell sheets.
Photothermally induced local dissociation of collagens for harvesting of cell sheets.
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DOI：
10.1002/anie.201411386
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发表时间：
2015-05
影响因子：
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通讯作者：
J. D. Kim;J. S. Heo;Teahoon Park;Chihyun Park;Hyun Ok Kim;Eunkyoung Kim
中科院分区：
文献类型：
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作者：
J. D. Kim;J. S. Heo;Teahoon Park;Chihyun Park;Hyun Ok Kim;Eunkyoung Kim
通过近红外（NIR）光引导的光热效应对聚（3，4-乙撑二氧噻吩）（PEDOT）进行局部加热，诱导吸收的胶原蛋白三螺旋展开，产生可溶性胶原蛋白单螺旋结构。胶原的这种解离允许在用NIR光（λ=808 nm）照射时获得活的自形细胞片。将PEDOT层图案化，并在图案化的基底上成功培养细胞。反映PEDOT图案的各种形状的细胞片可以在用NIR光照射几分钟后分离。PEDOT图案不仅引导电池片的整体形状，而且引导电池片中电池的展开方向。这种光热诱导的胶原蛋白解离提供了一种快速的非侵入性收获方法和定制的细胞片模式。
The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by a photothermal effect directed by near-infrared (NIR) light induces unfolding of absorbed collagen triple helices, yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of a living idiomorphic cell sheet, achieved upon irradiation with NIR light (λ=808 nm). The PEDOT layer was patterned and cells were successfully cultured on the patterned substrate. Cell sheets of various shapes mirroring the PEDOT pattern could be detached after a few minutes of irradiation with NIR light. The PEDOT patterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets. This photothermally induced dissociation of collagen provided a fast non-invasive harvesting method and tailor-made cell-sheet patterns.