Bone marrow stem cells contribute to repair of the ischemically injured renal tubule.
Bone marrow stem cells contribute to repair of the ischemically injured renal tubule.
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DOI：
10.1172/jci17856
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发表时间：
2003-07
期刊：
影响因子：
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通讯作者：
S. Kale;A. Karihaloo;P. R. Clark;M. Kashgarian;D. Krause;L. Cantley
中科院分区：
文献类型：
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作者：
S. Kale;A. Karihaloo;P. R. Clark;M. Kashgarian;D. Krause;L. Cantley
从急性肾小管坏死中恢复肾小管的范例是来自损伤边缘区域的存活细胞必须迁移到肾小管剥脱区域并增殖以重建正常的肾小管上皮。然而，旨在刺激这些事件的疗法在人体试验中未能改变急性肾衰竭的病程。在本研究中，我们证明，Lin-Sca-1+细胞从成年小鼠骨髓动员到循环短暂的肾缺血和家庭专门受伤的地区的肾小管。在那里，它们分化成肾小管上皮细胞，似乎构成了先前坏死的肾小管中存在的大部分细胞。骨髓消融后干细胞的丢失导致肾缺血后血尿素氮的更大升高，而骨髓消融后干细胞输注逆转了这一效应。因此，旨在增强骨髓干细胞向肾脏的动员、增殖和/或递送的疗法具有作为治疗急性肾小管坏死的全新方法的潜力。
The paradigm for recovery of the renal tubule from acute tubular necrosis is that surviving cells from the areas bordering the injury must migrate into the regions of tubular denudation and proliferate to re-establish the normal tubular epithelium. However, therapies aimed at stimulating these events have failed to alter the course of acute renal failure in human trials. In the present study, we demonstrate that Lin-Sca-1+ cells from the adult mouse bone marrow are mobilized into the circulation by transient renal ischemia and home specifically to injured regions of the renal tubule. There they differentiate into renal tubular epithelial cells and appear to constitute the majority of the cells present in the previously necrotic tubules. Loss of stem cells following bone marrow ablation results in a greater rise in blood urea nitrogen after renal ischemia, while stem cell infusion after bone marrow ablation reverses this effect. Thus, therapies aimed at enhancing the mobilization, propagation, and/or delivery of bone marrow stem cells to the kidney hold potential as entirely new approaches for the treatment of acute tubular necrosis.