A radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein.
A radiometric assay for determining the incorporation of L-canavanine or L-arginine into protein.
复制标题
用于确定 L-刀豆氨酸或 L-精氨酸掺入蛋白质的放射测定法。
DOI：
10.1016/0003-2697(85)90292-1
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发表时间：
1985
影响因子：
2.9
通讯作者：
Thomas,DA
中科院分区：
文献类型：
--
作者：
Rosenthal,GA;Thomas,DA
建立了L-[14C]鸟嘌呤核苷的放射分析方法，为新合成的蛋白质中[14C]刀豆碱的掺入提供了一种方便、准确的测量方法。这些方法也适用于蛋白质中[14C]精氨酸掺入的测定。用这些步骤研究了从两种破坏性强的害虫--六突圆线虫和绿色夜蛾的血淋巴中合成含L-[鸟嘌呤氧基-14C]刀豆氨酸和L-[鸟嘌呤-14C]精氨酸的蛋白质。
Procedures for a radiometric assay of l-[guanidinooxy-14C]canavanine were developed which provide a convenient and accurate measure of the incorporation of [14C]canavanine into de novo-synthesized proteins. These methods are also applicable to determining [14C]arginine incorporation into protein. These procedures have been employed to study the synthesis of l-[guanidinooxy-14C]canavanine- and l-[guanidino-14C]arginine-containing proteins from the hemolymph of Manduca sexta and Heliothis virescens, two highly destructive insect pests.