Chitosan/nano-hydroxyapatite/nano-zirconium dioxide scaffolds with miR-590-5p for bone regeneration
Chitosan/nano-hydroxyapatite/nano-zirconium dioxide scaffolds with miR-590-5p for bone regeneration
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DOI：
10.1016/j.ijbiomac.2018.01.122
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发表时间：
2018-05-01
影响因子：
8.2
通讯作者：
Selvamurugan, N.
中科院分区：
文献类型：
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作者：
Balagangadharan, K.;Chandran, S. Viji;Selvamurugan, N.
骨组织工程（BTE）依靠生物复合支架和生物活性分子进行骨再生。本研究旨在合成和表征包含壳聚糖（CS）、纳米羟基磷灰石（nHAp）和纳米二氧化锆（nZrO（2））以及用于 BTE 应用的 microRNA（miRNA）的生物复合材料支架。 miRNA 作为基因表达的转录后调节因子。使用 SEM、FT-IR 和 XRD 分析对制造的生物复合材料支架进行表征。使用小鼠间充质干细胞 (C3H10T1/2) 在细胞和分子水平上测试生物活性分子 (miR-590-5p) 与支架对成骨细胞分化的影响。结果表明，CS/nHAp/nZrO(2)支架促进成骨细胞分化，并且在C3H10T1/2细胞中存在miR-590-5p时，这种作用进一步增强。因此，我们认为带有miR-590-5p的CS/nHAp/nZrO(2)支架将具有治疗骨缺损的潜力。 (C) 2018 Elsevier B.V. 保留所有权利。
Bone tissue engineering (BTE) relies on biocomposite scaffolds and bioactive molecules for bone regeneration. The present study was aimed to synthesize and characterize biocomposite scaffolds containing chitosan (CS), nano-hydroxyapatite (nHAp) and nano zirconium dioxide (nZrO(2)) along with microRNA (miRNA) for BTE applications. miRNAs act as post-transcriptional regulator of gene expression. The fabricated biocomposite scaffolds were characterized using SEM, FT-IR and XRD analyses. The effect of a bioactive molecule (miR-590-5p) with scaffolds was tested for osteoblast differentiation at the cellular and molecular levels using mouse mesenchymal stem cells (C3H10T1/2). The results showed that CS/nHAp/nZrO(2) scaffolds promoted osteoblast differentiation, and this effect was further increased in the presence of miR-590-5p in C3H10T1/2 cells. Thus, we suggested that CS/nHAp/nZrO(2) scaffolds with miR-590-5p would have potential towards the treatment of bone defects. (C) 2018 Elsevier B.V. All rights reserved.