Vertical flow paper-based plasmonic device for cysteine detection
Vertical flow paper-based plasmonic device for cysteine detection
复制标题
DOI：
10.1007/s10544-019-0399-4
复制
发表时间：
2019-09-01
影响因子：
2.8
通讯作者：
D'Souza, Jacinta S.
中科院分区：
文献类型：
--
作者：
Mehta, Sourabh M.;Mehta, Shraddha;D'Souza, Jacinta S.
半胱氨酸尿症是一种常染色体隐性遗传性疾病，尿中难溶半胱氨酸水平越来越高，导致结石的形成。开发一种简便、低成本、方便、选择性的半胱氨酸诊断传感器势在必行。因此，对于半胱氨酸的检测，本研究展示了一种廉价的比色法、纸基垂直流动等离子体微井装置，其周转时间为两分钟。该方法包括使用微生物合成的银纳米颗粒(AgNPs)，当与半胱氨酸中存在的硫结合时，银纳米颗粒从浅棕色/黄色变成深棕色。这项技术允许视觉检测高达1x10(-5)mm的半胱氨酸，即使在资源最少的设置下，也可以很容易地作为快速诊断测试提供。
Cystinuria, is an autosomal recessive genetic disorder involving increasingly high levels of poorly soluble cysteine in urine leading to formation of stones. Developing a facile, low-cost, point-of-care and selective sensor for diagnosis of cysteine is imperative. Accordingly, for the detection of cysteine, the present study demonstrates an inexpensive colorimetric, paper-based vertical flow plasmonic micro-well device with a two-minute turn-around time. The method encompasses the use of microbially-synthesized silver nanoparticles (AgNPs) that change from light brown / yellow to dark brown upon binding with Sulphur present in cysteine. This technique allows for visual detection up to 1x10(-5)mM cysteine and can be easily offered as a rapid diagnostic test even at setups with minimal resources.