Infectivity of Cryptosporidiumandersoni Kawatabi type relative to thesmall number of oocysts in immunodeficient and immunocompetent neonatal and adult mice
Infectivity of Cryptosporidiumandersoni Kawatabi type relative to thesmall number of oocysts in immunodeficient and immunocompetent neonatal and adult mice
复制标题
安氏隐孢子虫川田型相对于免疫缺陷和免疫功能正常的新生和成年小鼠中少量卵囊的感染性
DOI：
10.1016/j.parint.2012.11.001
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sasai K
中科院分区：
文献类型：
--
作者：
Nagano-Koyashiki S;Matsubayashi M(CA);Kimata I;Furuya M;Tani H;Sasai K
安氏隐孢子虫是一种原生动物寄生虫，在许多国家发现，主要侵入成年牛的胃。与C. andersoni，C.从日本牛中分离的安氏梭菌能感染小鼠，被鉴定为一种新的类型，后来被定义为C. andersoni Kawatabi型。并对其生物学特性进行了研究。andersoni Kawatabi类型尚未得到很好的记载。在本研究中，我们评估了该类型分离株在不同免疫能力状态和年龄的小鼠中的感染性。我们发现，无论免疫状态如何，在成熟小鼠中建立感染需要接种超过1× 104个卵囊。所有感染的免疫活性小鼠在约20天的开放期后恢复。在免疫缺陷小鼠中，与1× 106个卵囊相比，开放前期延长，但以每天卵囊数量测量的模式和最大脱落几乎相同。在新生免疫活性小鼠和免疫缺陷小鼠中，也需要接种1× 104 ~ 105个卵囊才能建立感染。我们的研究结果表明，有一个阈值卵囊需要建立专利感染的酸性条件下的胃。
Cryptosporidium andersoni is a protozoan parasite found in many countries that invades the stomachs of primarily adult cattle. Unlike the isolates of C. andersoni in cattle from other countries, C. andersoni isolates from Japanese cattle can infect mice and were identified as a novel type and later defined as C. andersoni Kawatabi type. The biological characteristics of C. andersoni Kawatabi type have not yet been well documented. In the present study, we assess the infectivity of this type isolate in mice with different immune competence status and age. We found that inoculation of more than 1×104oocysts is needed to establish infection in mature mice irrespective of immune status. All of the infected immunocompetent mice recovered after a patent period of approximately 20days. In immunodeficient mice, the pre-patent period was prolonged compared with that of 1×106oocysts, but the pattern and the maximum shedding measured by the number of oocysts per day were almost identical. In neonatal immunocompetent and immunodeficient mice, inoculation with 1×104to 105oocysts was also needed to establish infection. Our results indicate that there is a threshold of oocysts needed to establish patent infection in the acidic conditions of the stomach.