Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae.
Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae.
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乙醇和蛋白质的乙醇植物副产品使用可食用的真菌神经孢子中间和曲曲霉。
DOI：
10.1155/2015/176371
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发表时间：
2015
影响因子：
--
通讯作者：
Lennartsson PR
中科院分区：
文献类型：
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作者：
Bátori V;Ferreira JA;Taherzadeh MJ;Lennartsson PR
生产第二代乙醇的可行生物精炼厂由于工艺复杂而难以建立。一种替代办法是在第一代工厂中部分采用这一工艺。酒糟是谷物干磨乙醇工艺的副产品，主要由未降解的麸皮和木质纤维素组成，可用作生产乙醇和饲料蛋白的潜在底物。以中间脉孢菌和曲霉为发酵菌种，研究了酒精发酵和酒糟中蛋白质的提取。N. Intermedia从釜馏物产生4.7g/L乙醇，并且通过添加lFPU的纤维素酶/g悬浮固体而增加到8.7g/L。酿酒酵母分别产生0.4和5.1 g/L乙醇。在两种真菌的两阶段培养下，获得了高达7.6g/L的乙醇和5.8g/L的生物量（含42%（w/w）的粗蛋白）。这两种真菌降解复杂的底物，包括阿拉伯聚糖，葡聚糖，甘露聚糖，木聚糖，其中减少91，73，38和89%（w/v），分别实现。采用当前工艺可生产44，000 m3乙醇（提高22%），约12，000吨富含蛋白质的生物质用于动物饲料，并节省能源，考虑到典型的设施每年生产200，000 m3乙醇。
Feasible biorefineries for production of second-generation ethanol are difficult to establish due to the process complexity. An alternative is to partially include the process in the first-generation plants. Whole stillage, a by-product from dry-mill ethanol processes from grains, is mostly composed of undegraded bran and lignocelluloses can be used as a potential substrate for production of ethanol and feed proteins. Ethanol production and the proteins from the stillage were investigated using the edible fungi Neurospora intermedia and Aspergillus oryzae, respectively. N. intermedia produced 4.7 g/L ethanol from the stillage and increased to 8.7 g/L by adding 1 FPU of cellulase/g suspended solids. Saccharomyces cerevisiae produced 0.4 and 5.1 g/L ethanol, respectively. Under a two-stage cultivation with both fungi, up to 7.6 g/L of ethanol and 5.8 g/L of biomass containing 42% (w/w) crude protein were obtained. Both fungi degraded complex substrates including arabinan, glucan, mannan, and xylan where reductions of 91, 73, 38, and 89% (w/v) were achieved, respectively. The inclusion of the current process can lead to the production of 44,000 m3 of ethanol (22% improvement), around 12,000 tons of protein-rich biomass for animal feed, and energy savings considering a typical facility producing 200,000 m3 ethanol/year.