Cloning and sequence analysis of genes encoding Staphylococcus hyicus exfoliative toxin types A, B, C, and D
Cloning and sequence analysis of genes encoding Staphylococcus hyicus exfoliative toxin types A, B, C, and D
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DOI：
10.1128/jb.186.6.1833-1837.2004
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发表时间：
2004-03-01
影响因子：
3.2
通讯作者：
Andresen, LO
中科院分区：
文献类型：
--
作者：
Ahrens, P;Andresen, LO
猪葡萄球菌某些菌株产生的脱落毒素可介导猪渗出性表皮炎。本研究对猪链霉菌的四种不同脱落毒素(ExhA、ExhB、ExhC和ExhD)的编码基因进行了克隆和测序。4个毒力基因的编码序列在816~834bp之间。这四种毒素的氨基酸序列与早先报道的金黄色葡萄球菌脱落毒素SHETB和金黄色葡萄球菌的ETA、ETB和ETD同源。海链球菌毒素与金黄色葡萄球菌脱落毒素的同源性在同一水平。这些毒素显示出与丝氨酸蛋白酶的相似之处，包括在ExhA、ExhB和ExhC中保留了催化道。然而，在ExhD中，推测的催化道中的天冬氨酸被Glu取代。重组毒素可在大肠杆菌中表达，4种毒素中的3种可被抗天然脱落毒素的单抗识别。
Exfoliative toxins produced by certain strains of Staphylococcus hyicus mediate exudative epidermitis in pigs. In this study the genes coding for four different exfoliative toxin from S. hyicus (ExhA, ExhB, ExhC, and ExhD) were cloned and sequenced. The coding sequence of the four toxin genes ranged from 816 to 834 bp. The amino acid sequences of these four toxins were homologous to the earlier described exfoliative toxins SHETB from S. hyicus and ETA, ETB, and ETD from Staphylococcus aureus. The homology between the S. hyicus toxins was at the same level as the homology to the exfoliative toxins from S. aureus. The toxins showed similarity to serine proteases, including preservation of the catalytic tract in ExhA, ExhB, and ExhC. However, in ExhD, Asp in the putative catalytic tract was replaced with Glu. The recombinant toxins could be expressed in Escherichia coli, and three of the four toxins were recognized by monoclonal antibodies raised against native exfoliative toxins.