Altered somatosensory cortex neuronal activity in a rat model of Parkinson's disease and levodopa-induced dyskinesias
Altered somatosensory cortex neuronal activity in a rat model of Parkinson's disease and levodopa-induced dyskinesias
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DOI：
10.1016/j.expneurol.2017.04.011
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发表时间：
2017-08-01
影响因子：
5.3
通讯作者：
Schwabe, Kerstin
中科院分区：
文献类型：
--
作者：
Alam, Mesbah;Rumpel, Regina;Schwabe, Kerstin
几项研究结果支持的概念，感觉运动整合在帕金森病（PD）和左旋多巴诱导的运动障碍。在这项研究中，我们探讨了初级躯体感觉皮层前肢区（S1 FL-Ctx）兴奋性锥体细胞和抑制性中间神经元的神经元放电活动，沿着其与初级运动皮层（MCtx）振荡活动的相互作用，与6-羟基多巴胺损伤的偏侧帕金森病（HP）和左旋多巴引发的运动障碍（HP-LID）大鼠相比，乌拉坦（1.4kg/lcg，i. p.）麻醉此外，抑制性GABA能神经元的不同标记物的基因表达模式进行了分析，在两个cortex regions.While放电频率和爆发活动的S1 FL-Ctx抑制性中间神经元减少HP和HP-LID大鼠，不规则的措施，在锥体细胞增强。此外，观察到θ/α、高β和γ频率的不同频带的相干性增强，以及推定的锥体细胞和中间神经元与MCtx振荡活性的同步性增强。虽然GABA水平相似，但HP-LID大鼠的S1 FL-Ctx和MCtx中的中间神经元和GABA能标记物的基因表达水平存在一定程度的差异。我们的研究表明，在运动障碍的PD大鼠模型中，假定的中间神经元中的神经元活性降低，这伴随着S1 FL-Ctx和MCtx之间的高β和γ相干性，以及基因表达的变化，表明长期左旋多巴治疗后的适应不良神经可塑性。(C)2017爱思唯尔公司All rights reserved.
Several findings support the concept that sensorimotor integration is disturbed in Parkinson's disease (PD) and in levodopa-induced dyskinesias. In this study, we explored the neuronal firing activity of excitatory pyramidal cells and inhibitory interneurons in the forelimb region of the primary somatosensory cortex (S1FL-Ctx), along with its interaction with oscillatory activity of the primary motor cortex (MCtx) in 6-hydroxydopamine lesioned hemiparkinsonian (HP) and levodopa-primed dysldnetic (HP-LID) rats as compared to controls under urethane (1.4kg/lcg, i.p.) anesthesia. Further, gene expression patterns of distinct markers for inhibitory GABAergic neurons were analyzed in both cortical regions.While firing frequency and burst activity of S1FL-Ctx inhibitory interneurons were reduced in HP and HP-LID rats, measures of irregularity were enhanced in pyramidal cells. Further, enhanced coherence of distinct frequency bands of the theta/alpha, high-beta, and gamma frequency, together with enhanced synchronization of putative pyramidal cells and interneurons with MCtx oscillatory activity were observed. While GABA level was similar, gene expression levels of interneuron and GABAergic markers in S1FL-Ctx and MCtx of HP-LID rats differed to some extent.Our study shows that in a rat model of PD with dyskinesias, neuronal activity in putative interneurons was reduced, which was accompanied by high beta and gamma coherence between S1FL-Ctx and MCtx, together with changes in gene expression, indicating maladaptive neuroplasticity after long term levodopa treatment. (C) 2017 Elsevier Inc. All rights reserved.