Genes with specific functions in the ovarian follicles of Calliphora erythrocephala (Diptera).
Genes with specific functions in the ovarian follicles of Calliphora erythrocephala (Diptera).
复制标题
红头丽丽（双翅目）卵巢卵泡中具有特定功能的基因。
DOI：
10.1016/0012-1606(88)90392-2
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发表时间：
1988
影响因子：
2.7
通讯作者：
Beckingham,K
中科院分区：
文献类型：
--
作者：
Rubacha,A;Tucker,MA;deValoir,T;Belikoff,EJ;Beckingham,K
工作与大dipteranCalliphora红头丽蝇，我们进行了差异筛选，以确定在previtellogenic和早期卵黄发生阶段的卵子发生，但沉默的早期阶段的胚胎发生中积极表达的基因。含有与四个这样的基因同源的序列的克隆已经被表征。其中两个克隆与果蝇卵黄蛋白1基因同源。这两个克隆不仅在卵母细胞周围的柱状卵泡细胞中表达，而且在卵泡细胞的高度特化亚群--边缘细胞中也表达。这表明了这些细胞的一种新功能，以前认为这些细胞主要参与珠孔的形成。第三个克隆与D.在染色体基因座26 A处簇的黑色素卵黄膜蛋白基因仅在卵周卵泡细胞中表达，而不在边缘细胞群中表达。第四个克隆编码一个未知功能的序列，该序列在卵泡的生殖系细胞中大量表达。与该克隆同源的转录物持续存在于成熟卵泡中，最初集中在卵母细胞的前极。重复DNA在这4个克隆中的分布表明，C.红细胞基因组具有重复DNA的短散布排列。
Working with the large dipteranCalliphora erythrocephala, we have performed differential screening to identify genes actively expressed in the previtellogenic and early vitellogenic stages of oogenesis but silent during the early stages of embryogenesis. Clones containing sequences homologous to four such genes have been characterized. Two clones are homologous to the yolk protein 1 gene ofDrosophila melanogaster. These two clones are expressed not only in the columnar follicle cells surrounding the oocyte but also in the border cells—a highly specialized subgroup of the follicle cells. This indicates a new function for these cells thought previously to contribute mainly in the formation of the micropyle. A third clone, which is related to theD. melanogastervitelline membrane protein genes of the cluster at chromosomal locus 26A, is expressed only in the perioocyte follicle cells and not the border cell population. The fourth clone encodes a sequence of unknown function which is abundantly expressed in the germ line cells of the follicle. Transcripts homologous to this clone persist into the mature follicle and initially appear concentrated at the anterior pole of the oocyte. The distribution of repetitious DNA within these four clones indicates that theC. erythrocephalagenome has a short interspersion arrangement of repetitive DNA.