Genetic diversity and structure in Escherichia coli populations.
Genetic diversity and structure in Escherichia coli populations.
复制标题
大肠杆菌种群的遗传多样性和结构。
DOI：
10.1126/science.6999623
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发表时间：
1980
期刊：
影响因子：
56.9
通讯作者：
B. Levin
中科院分区：
文献类型：
--
作者：
R. Selander;B. Levin
从109个克隆的大肠杆菌从自然人群中的20种酶的电泳变异的调查产生的平均遗传多样性的估计约两倍，在早期的研究报告和4至5倍，比大多数真核生物物种的估计。尽管存在这种广泛的变异性，但独特基因型的数量显然是相当有限的。从无关宿主中获得相同的克隆，并从人类婴儿中分离出一个与实验室菌株大肠杆菌K-12在细菌学上无法区分的克隆。结果表明，大肠杆菌自然群体中的遗传重组率很低。这些发现对我们理解大肠杆菌种群的遗传结构和决定该细菌物种中性基因变异量的因素具有影响。
A survey of electrophoretic variation in 20 enzymes from 109 clones of escherichia coli from natural populations yielded an estimate of mean genetic diversity approximately twice that reported in an earlier study and four to five times larger than estimates fro most eukaryotic species. Despite this extensive variability, the number of distinctive genotypes apparently is rather limited. Identical clones were obtained from unassociated hosts, and a clone that is electrophoretically indistinguishable from the laboratory strain Escherichia coli K-12 was isolated from a human infant. The results suggest that rates of genetic recombination in natural populations of Escherichia coli are low. These findings have implications for our understanding of the genetic structure of Escherichia coli populations and the factors determining the amount of neutral gene variability in this bacterial species.