Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy
Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy
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DOI：
10.1016/j.jconrel.2003.11.012
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发表时间：
2004-03-05
影响因子：
10.8
通讯作者：
Mäder, K
中科院分区：
文献类型：
--
作者：
Jores, K;Mehnert, W;Mäder, K
最近，由固体和液体脂质的混合物制成的胶体分散体被描述为将联合收割机控制释放特性与比固体脂质纳米颗粒（SLN）更高的药物负载能力相结合。已经提出，这些纳米结构脂质载体（NLC）由油滴组成，所述油滴溶解药物并且包埋在固体脂质基质中。采用光子相关光谱（PCs）、激光衍射（LD）、场流分级分离（FFF）、多角度光散射检测（MALS）和低温透射电子显微镜（cryo TEM）等方法对以山嵛酸甘油酯和中链甘油三酯为原料的SLN和NLC的结构进行了表征。PC表明SLN和NLC由于不对称的颗粒形状而在布朗运动方面不同于纳米乳液。在SLN和NLC的情况下，非球形颗粒导致与纳米乳液相比更高的多分散性指数。在FFF中，纳米液滴比SLN-和NLC-血小板早得多，尽管它们的PC和LD数据显示出相似的粒度。在TEM片晶（对于SLN）中，观察到载油片晶（“纳米勺”;对于NLC）和液滴（对于纳米乳液）结构。与文献报道相反，研究的SLN表现为薄的血小板。发现NLC是表面粘附有油点的脂质血小板。与常规纳米乳剂相比，SLN和NLC在血小板中的扩散途径非常短、水-脂质界面增加和结晶脂质中的药物掺入低是SLN和NLC的缺点。(C)2004 Elsevier B. V.保留所有权利。
Recently, colloidal dispersions made from mixtures of solid and liquid lipids were described to combine controlled release characteristics with higher drug loading capacities than solid lipid nanoparticles (SLN). It has been proposed that these nanostructured lipid carriers (NLC) are composed of oily droplets which solubilize the drug and which are embedded in a solid lipid matrix. The structures of SLN and NLC based on glyceryl behenate and medium chain triglycerides were characterized by photon correlation spectroscopy (PCs) and laser diffraction (LD), field-flow fractionation (FFF) with multi-angle light scattering detection (MALS), and cryo transmission electron microscopy (cryo TEM). PCs indicates that SLN and NLC differ from a nanoemulsion with respect to Brownian motion due to asymmetric particle shapes. Non-spherical particles, in case of SLN and NLC, lead to higher polydispersity indices compared to the nanoemulsion. In FFF, the nanodroplets elute much earlier than SLN- and NLC-platelets although their PCs and LD data show similar particle sizes. In TEM platelet (for SLN), oil loaded platelet ("nanospoons"; for NLC) and droplet (for nanoemulsion) structures were observed. In contrast to literature reports, the investigated SLN appear as thin platelets. NLC are found to be lipid platelets with oil spots sticking on the surface. Very short diffusion pathways in platelets, increased water-lipid interfaces and low drug incorporation in crystalline lipids are the drawback of SLN and NLC compared to conventional nanoemulsions. (C) 2004 Elsevier B.V. All rights reserved.