The mechanisms of PM2.5 and its main components penetrate into HUVEC cells and effects on cell organelles
The mechanisms of PM2.5 and its main components penetrate into HUVEC cells and effects on cell organelles
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PM2.5及其主要成分渗入HUVEC细胞的机制及对细胞器的影响
DOI：
10.1016/j.chemosphere.2019.125127
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发表时间：
2020-02-01
期刊：
影响因子：
8.8
通讯作者：
Li, Zhuoyu
中科院分区：
文献类型：
--
作者：
Su, Ruijun;Jin, Xiaoting;Li, Zhuoyu
大气颗粒物（PM2.5）与心血管疾病的发病率和死亡率有关。然而，PM2.5是否会渗透到细胞中以及潜在的机制尚不清楚。因此，本研究首次表明PM2.5可以渗入HUVEC细胞，吞噬作用、微胞吞噬作用、小泡蛋白和网格蛋白介导了PM2.5进入HUVEC细胞的内化。其中，PM2.5-Metal、PAHs和WSC主要通过微胞吞作用、网格蛋白和小洞蛋白介导的内吞作用进入HUVEC细胞。目前的环境评价数据表明，PM2.5-Metal对生态环境和人体健康危害极大。此外，PM2.5和/或PM2.5- metal处理的HUVEC细胞中，线粒体融合基因Mfn1增加，裂变基因Opa1和Drp1减少，溶酶体相关基因LAMP2和LAMP3减少，线粒体和溶酶体形态均出现损伤现象。这些数据表明PM2.5及其主要成分通过不同的内吞作用进入HUVEC细胞并引起线粒体和溶酶体损伤。因此，我们的研究提供了雾霾颗粒渗入HUVEC细胞并破坏细胞器的潜在机制。(C) 2019年Elsevier Ltd.出版
Atmospheric particulate matter (PM2.5) is associated with the morbidity and mortality of cardiovascular diseases. However, whether PM2.5 penetrates into the cells and the potential mechanisms are unknown. Hence, the study firstly indicated that PM2.5 could penetrate into the HUVEC cells, and phagocytosis, micropinocytosis, caveolin as well as clathrin mediated the internalization of PM2.5 into HUVEC cells. Particularly, the components of PM2.5-Metal, PAHs and WSC could enter into HUVEC cells mainly via the micropinocytosis, clathrin and caveolin mediated endocytosis, respectively. The current data of environmental assessments indicated that PM2.5-Metal were extremely harmful to the ecological environment and human health. Moreover, accompanying with mitochondrial fusion gene Mfn1 was increased and fission genes Opa1 and Drp1 were decreased, and the lysosome related genes LAMP2 and LAMP3 were decreased, the phenomenon that the morphology of mitochondrial and lysosome injured was observed in HUVEC cells treated with PM2.5 and/or PM2.5-Metal. These data suggest that PM2.5 and its main components depend on different endocytosis penetrate into HUVEC cells and cause the mitochondrial and lysosomal damages. Thereby, our study provides the potential mechanism of haze particles penetration into HUVEC cells and damage to organelles. (C) 2019 Published by Elsevier Ltd.