Transcriptional control of two ribosome-inactivating protein genes expressed in spinach (Spinacia oleracea) embryos
Transcriptional control of two ribosome-inactivating protein genes expressed in spinach (Spinacia oleracea) embryos
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DOI：
10.1016/j.plaphy.2008.12.020
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发表时间：
2009-05-01
影响因子：
6.5
通讯作者：
Masuda, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kawade, Kensuke;Masuda, Kiyoshi
SoRIP1 和 SoRIP2 是在菠菜 (Spinacia oleracea) 中鉴定的核糖体失活蛋白 (RIP: EC 3.2.2.22) 基因。它们在菠菜体细胞胚胎发生过程中以发育依赖性方式差异表达。在这里，我们分离了 SoRIP1 和 SoRIP2 的基因组克隆。这两个 RIP 基因具有不同的基因组组织。对石竹目植物中 RIP 的预测氨基酸序列进行的系统发育分析表明，它们分为两个主要亚科，分别对应于 SoRIP1 和 SoRIP2。为了进一步了解 SoRIP1 和 SoRIP2 的转录控制，我们通过反向 PCR 获得了它们的 5' 侧翼序列。两个 5' 侧翼序列的比较揭示了每个区域中赋予差异转录控制的特征顺式元件。在SoRIP1的5'侧翼区域，我们发现了几个具有与胚胎发育相关功能的基序。 SoRIP2 的 5' 侧翼区域包含一些防御响应基序。在多种组织中检测到 SoRIP1 的表达。特别是，SoRIP1在早期未成熟果实中高表达，免疫组化显示SoRIP1在未成熟胚的外围区域积累，在内部细胞中表达较弱。在果实发育过程中，SoRIP2的表达量较低。然而，SoRIP2在未成熟胚胎的表皮中的积累是明显的。水杨酸处理诱导叶片中 SoRIP2 的表达，但不诱导 SoRIP1 的表达。 SoRIP1和SoRIP2的这种差异转录调控表明相应的蛋白质可能具有不同的功能，一个与胚胎发育相关，另一个与胚胎防御相关。 (C) 2009 Elsevier Masson SAS。版权所有。
SoRIP1 and SoRIP2 are ribosome-inactivating protein (RIP: EC 3.2.2.22) genes identified in spinach (Spinacia oleracea). They are differentially expressed in a development-dependent manner during spinach somatic embryogenesis. Here, we isolated genomic clones of SoRIP1 and SoRIP2. These two RIP genes have different genomic Organization. Phylogenetic analysis Of predicted amino acid sequences of RIPs in Caryophyllales plants revealed that they are divided into two major subfamilies, corresponding to SoRIP1 and SoRIP2. To gain further insight into the transcriptional control of SoRIP1 and SoRIP2, we obtained their 5'-flanking sequences by inverse PCR. Comparison of two 5'-flanking sequences revealed the characteristic cis elements in each region that confer differential transcriptional control. In the 5'-flanking region of SoRIP1, we found several motifs with functions related to embryonic development. The 5'-flanking region of SoRIP2 contains some defense-responsive motifs. Expression of SoRIP1 was detected in various tissues. In Particular, SoRIP1 was highly expressed in the early immature fruits, and immunohistochemistry showed that SoRIP1 accumulated in the peripheral region of the immature embryo, with weaker expression in internal cells. During fruit development, the expression of SoRIP2 was low. However, the accumulation of SoRIP2 was conspicuous in the epidermis of the immature embryo. The expression of SoRIP2, but not SoRIP1, in leaves was induced by salicylic acid treatment. This differential transcriptional regulation of SoRIP1 and SoRIP2 suggests that the corresponding proteins may have different functions, one being related to embryonic development and the other to embryo defense. (C) 2009 Elsevier Masson SAS. All rights reserved.