Loss of Bladder Epithelium Induced by Cytolytic Mast Cell Granules.
Loss of Bladder Epithelium Induced by Cytolytic Mast Cell Granules.
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DOI：
10.1016/j.immuni.2016.11.003
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发表时间：
2016-12-20
期刊：
影响因子：
32.4
通讯作者：
Abraham, Soman N.
中科院分区：
文献类型：
--
作者：
Choi, Hae Woong;Bowen, Samantha E.;Miao, Yuxuan;Chan, Cheryl Y.;Miao, Edward A.;Abrink, Magnus;Moeser, Adam J.;Abraham, Soman N.
上皮细胞的程序性死亡和脱落是减少感染过程中细菌负荷的一种强大的防御机制，但由于上皮细胞的关键屏障功能，这种活动不能不加区别地进行。我们报道，在膀胱炎期间，在受感染的膀胱上皮细胞(BECs)脱落之前，肥大细胞(MCs)直接在表层上皮下募集，在那里它们停靠并挤出颗粒。MCs对炎症小体和半胱氨酸天冬氨酸蛋白酶-1信号传导后分泌的白介素1β(IL-1β)有反应。当BECs摄取颗粒相关的糜酶(小鼠MC蛋白水解酶4(MMCPT4))时，BECs经历caspase-1相关的细胞溶解和脱落。因此，受感染的上皮细胞在脱落之前需要从重新招募的前哨炎症细胞中获得特定的细胞溶解提示。Choi等人。证明肥大细胞介导了膀胱上皮细胞(BECs)的脱落，这是感染过程中一种强大的防御机制。感染后，BEC以炎症依赖的方式分泌IL-1β，将肥大细胞招募到表面上皮细胞。被激活的肥大细胞释放含有糜酶的颗粒，当被BECs摄取时，触发caspase-1介导的细胞溶解。
Programmed death and shedding of epithelial cells is a powerful defense mechanism to reduce bacterial burden during infection but this activity cannot be indiscriminate because of the critical barrier function of the epithelium. We report that during cystitis, shedding of infected bladder epithelial cells (BECs) was preceded by the recruitment of mast cells (MCs) directly underneath the superficial epithelium where they docked and extruded their granules. MCs were responding to interleukin-1β (IL-1β) secreted by BECs following inflammasome and caspase-1 signaling. Upon uptake of granule-associated chymase (mouse MC protease 4 (mMCPT4)), BECs underwent caspase-1 associated cytolysis and exfoliation. Thus, infected epithelial cells require a specific cue for cytolysis from recruited sentinel inflammatory cells before shedding. Choi et al. demonstrate that mast cells mediate bladder epithelial cell (BECs) exfoliation, a powerful defense mechanism during infection. Following infection, BECs secrete IL-1β in an inflammasome-dependent manner, which recruits mast cells to the superficial epithelium. Activated mast cells release chymase-containing granules which upon uptake by BECs trigger caspase-1 mediated cytolysis.
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DOI：
10.4049/jimmunol.1300023
发表时间：
2013-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Subramanian H;Gupta K;Lee D;Bayir AK;Ahn H;Ali H
通讯作者：
Ali H
影响因子：
64.8
作者：
Malaviya, R;Ikeda, T;Abraham, SN
通讯作者：
Abraham, SN
影响因子：
5.1
作者：
Oliveira, S. H. P.;Canetti, C.;Cunha, F. Q.
通讯作者：
Cunha, F. Q.
DOI：
10.1073/pnas.1500374112
发表时间：
2015-02-24
影响因子：
11.1
作者：
Nagamatsu, Kanna;Hannan, Thomas J.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
影响因子：
30.3
作者：
Mysorekar IU;Isaacson-Schmid M;Walker JN;Mills JC;Hultgren SJ
通讯作者：
Hultgren SJ