Increased Levels of ɛ and γ Isoforms of 14-3-3 Proteins in Cerebrospinal Fluid in Patients with Creutzfeldt-Jakob Disease
Increased Levels of ɛ and γ Isoforms of 14-3-3 Proteins in Cerebrospinal Fluid in Patients with Creutzfeldt-Jakob Disease
复制标题
克雅氏病患者脑脊液中 14-3-3 蛋白 ɛ 和 γ 亚型水平升高
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
T. Kurata
中科院分区：
文献类型：
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作者：
Hidehiro Takahashi;T. Iwata;Y. Kitagawa;R. Takahashi;Yuko Sato;H. Wakabayashi;M. Takashima;H. Kido;K. Nagashima;K. Kenney;C. Gibbs;T. Kurata
我们建立了4株抗14-3-3蛋白的单克隆抗体杂交瘤细胞系。免疫印迹分析显示，在散发性克雅氏病患者的死前脑脊液样本中，α和γ亚型特异性增加。斑点免疫印迹分析表明，单克隆抗体对天然抗原的特异性高于多克隆抗体。
ABSTRACT We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that ɛ and γ isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.
影响因子：
158.5
作者：
Hsich, G;Kinney, K;Harrington, MG
通讯作者：
Harrington, MG