A method of liberating living cells from the dermal infiltrate
A method of liberating living cells from the dermal infiltrate
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一种从真皮浸润中释放活细胞的方法
DOI：
10.1111/j.1365-2133.1975.tb06492.x
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发表时间：
1975
影响因子：
10.3
通讯作者：
J. Hayes
中科院分区：
文献类型：
--
作者：
R. Tan;N. Byrom;J. Hayes
描述了一种新的技术，通过这种技术，可以从皮肤活检标本中分离出有活力的浸润性细胞。标本与胶原酶孵育，然后机械分解。获得的细胞仍可用于免疫学和形态学研究。用这种方法比较了皮肤网状增生症和扁平苔藓患者真皮浸润物的性质。真菌样肉芽肿、S综合征和扁平苔藓中T细胞占优势，非霍奇金淋巴瘤中B细胞占优势。
A new technique is described whereby viable infiltrating cells can be freed from skin biopsy specimens. The specimens are incubated with collagenase and then mechanically disaggregated. The liberated cells are still suitable for immunological and morphological study. Using this method, the nature of the dermal infiltrate in patients with skin reticuloses was compared with that in lichen planus. A predominance of T cells was found in mycosis fungoides, the Sézary syndrome, and lichen planus, and of B cells in non‐Hodgkin lymphomas.