Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus.
Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus.
复制标题
人类 DNA 序列与莫洛尼鼠肉瘤病毒的转化基因 (mos) 同源。
DOI：
--
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
G. V. Vande Woude
中科院分区：
文献类型：
--
作者：
R. Watson;M. Oskarsson;G. V. Vande Woude
我们描述的分子克隆的9千碱基对BamHI片段从人胎盘DNA含有同源序列的莫洛尼鼠肉瘤病毒的转化基因（V-MOS）。解析人DNA（称为humos）的同源区的DNA序列，并与v-mos（称为mumos）的小鼠细胞同源物的DNA序列进行比较[货车贝弗伦，C.，货车Straaten，F.，Gallesville，J.A. & Verma，I.M.（1981）Cell 27，97-108]。humos基因含有346个密码子的开放阅读框，通过在mumos DNA中引入15和3个碱基的两个缺口以及在humos DNA中引入9个碱基的单个缺口，将其与等同的mumos DNA序列进行比对。比对的编码序列有77%同源性，并终止于等价的蛋白石密码子。humos开放阅读帧在mumos编码序列内部发现的ATG处启动。从DNA序列中预测的由humos和mumos编码的多肽也被发现是广泛同源的，337个氨基酸中有253个是两种多肽共有的。humos基因产物的前5个NH 2-末端和最后2个COOH-末端氨基酸与mumos基因产物的氨基酸相同。此外，在多肽链的中间附近，范围从19至26个连续氨基酸的四个区域是保守的。然而，我们还不能用转染的humos DNA片段或含有humos和逆转录病毒长末端重复序列（LTR）序列的杂交DNA重组体转化小鼠细胞。
We describe the molecular cloning of a 9-kilo-base-pair BamHI fragment from human placental DNA containing a sequence homologous to the transforming gene (v-mos) of Moloney murine sarcoma virus. The DNA sequence of the homologous region of human DNA (termed humos) was resolved and compared to that of the mouse cellular homolog of v-mos (termed mumos) [Van Beveren, C., van Straaten, F., Galleshaw, J.A. & Verma, I.M. (1981) Cell 27, 97-108]. The humos gene contained an open reading frame of 346 codons that was aligned with the equivalent mumos DNA sequence by the introduction of two gaps of 15 and 3 bases into the mumos DNA and a single gap of 9 bases into the humos DNA. The aligned coding sequences were 77% homologous and terminated at equivalent opal codons. The humos open reading frame initiated at an ATG found internally in the mumos coding sequence. The polypeptides predicted from the DNA sequence to be encoded by humos and mumos also were found to be extensively homologous, and 253 of 337 amino acids were shared between the two polypeptides. The first five NH2-terminal and last two COOH-terminal amino acids of the humos gene product were in common with those of mumos. In addition, near the middle of the polypeptide chains, four regions ranging from 19 to 26 consecutive amino acids were conserved. However, we have not been able to transform mouse cells with transfected humos DNA fragments or with hybrid DNA recombinants containing humos and retroviral long terminal repeat (LTR) sequences.