Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio).
Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio).
复制标题
DOI：
10.1039/c8fo02109k
复制
发表时间：
2019-02
期刊：
影响因子：
6.1
通讯作者：
Mohamed A. Gedi;K. Magee;Randa Darwish;P. Eakpetch;Iain S. Young;D. Gray
中科院分区：
文献类型：
--
作者：
Mohamed A. Gedi;K. Magee;Randa Darwish;P. Eakpetch;Iain S. Young;D. Gray
全球水产养殖产量的增加对鱼粉和鱼油供应造成了压力。因此，人们越来越多地寻找更可持续的蛋白质和多不饱和脂肪酸来源作为鱼类饲料成分。叶绿体是植物叶片中的细胞器，许多有价值的营养物质，脂肪酸（FAs）、氨基酸、维生素和色素都是在叶绿体中合成的。叶绿体可以加入鱼的饲料中，要么保留在植物细胞中，要么从植物细胞中释放出来。在这项研究中，斑马鱼分别被喂食7种不同的食物；饲料中添加降低鱼粉水平（10%、20%或50%）的菠菜叶粉（SLP）或通过既定方法制备的富含叶绿体的组分（CRF）来回收叶绿体。SLP和CRF对鱼的生长、味觉反应、全鱼FA组成和类胡萝卜素均有积极影响。CRF饲料中α-亚麻酸（C18:3 n-3）和十六烯酸（C16:3）含量显著（P≤0.05）高于SLP和对照组。十六烷酸（C16:3）是叶绿体半乳糖脂中独特的FA；它在斑马鱼组织中的存在证明斑马鱼消化和吸收叶绿体半乳糖脂。CRF饲料对斑马鱼卵叶黄素含量的影响显著（P≤0.05）高于SLP饲料和对照组。还注意到鸡蛋颜色的变化，需要进一步研究饮食对鱼类繁殖力、胚胎繁殖力、孵化率和幼虫存活率的影响。
Increasing global aquaculture production, is putting pressure on fishmeal and fish oil supply. There is therefore a growing search for more sustainable sources of proteins and polyunsaturated fatty acids as fish feed ingredients. Chloroplasts are the organelles in the leaves of plants where many of the valuable nutrients, fatty acids (FAs), amino acids, vitamins and pigments, are synthesised. Chloroplasts could be incorporated into fish diets either retained in, or liberated from, plant cells. In this study zebrafish were fed with seven different diets individually; fish were fed with diets reducing fishmeal levels (10, 20 or 50%) using either spinach leaf powder (SLP) or a chloroplast rich fraction (CRF) prepared by an established method to recover chloroplasts. Both SLP and CRF had a positive impact on the growth, taste response, whole fish FA composition, and carotenoid profile. Fish fed with CRF diets showed significantly (P ≤ 0.05) greater α-linolenic (C18:3 n-3) and hexadecatrienoic (C16:3) acid contents than those of SLP and the control. Hexadecanoic acid (C16:3) is a unique FA in the galactolipids of the chloroplast; its presence in zebrafish tissues proves that zebrafish digest and absorb chloroplast galactolipids. The lutein profile of eggs produced by zebrafish fed with the CRF diet was significantly (P ≤ 0.05) higher than those of SLP and the control. Alterations in egg colour were also noted, warranting further investigations of the diet impact on fish fecundity, embryo fertility, hatch rate and larval survival.