Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardtii. II: Purification and characterization of minus agglutinin and comparison with plus agglutinin
Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardtii. II: Purification and characterization of minus agglutinin and comparison with plus agglutinin
复制标题
莱茵衣藻交配型负配子的性凝集素。
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Y. Matsuda
中科院分区：
文献类型：
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作者：
Tatsuaki Saito;Y. Matsuda
用凝胶过滤和羟基磷灰石层析法从莱茵衣藻交配型负配子中纯化出性凝集素。经SDS-聚丙烯酰胺凝胶电泳鉴定，该负凝集素是一个分子量很高的糖多肽，称为Agg（-）。在非SDS聚丙烯酰胺凝胶上也观察到其为具有凝集素活性的糖蛋白。天然凝集素似乎由Agg（-）亚基复合物组成。它由大约60%的蛋白质和40%的碳水化合物组成，并且活性被轻度的高碘酸盐氧化所减弱。将正凝集素与负凝集素进行比较，发现两者在SDS-聚丙烯酰胺凝胶电泳中的迁移位置相同，而在凝胶过滤和羟基磷灰石层析中的行为不同。
The sexual agglutinin from the mating-type minus gametes ofChlamydomonas reinhardtii was purified by gel filtration and hydroxyapatite chromotography. The minus agglutinin was identified as a single glycopolypeptide termed Agg(-) of very high molecular weight by SDS-poly-acrylamide gel electrophoresis. It was also observed as a glycoprotein with agglutinin activity on non-SDS polyacrylamide gels. The native agglutinin appeared to be composed of a complex of Agg(-) subunits. It consisted of about 60% protein and 40% carbohydrate and the activity was diminished by a mild periodate oxidation. When the plus agglutinin was also purified and compared with the minus agglutinin, it was found that both agglutinins migrate in the same position by SDS-polyacrylamide gel electrophoresis, whereas their behaviors on gel filtration and hydroxyapatite chromatography are different.
来自衣藻鞭毛膜的性凝集素。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Adair,WS;Monk,BC;Cohen,R;Hwang,C;Goodenough,UW
通讯作者：
Goodenough,UW