Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction.
Identification of selected gamma-ray induced deficiencies in zebrafish using multiplex polymerase chain reaction.
复制标题
使用多重聚合酶链反应鉴定斑马鱼中选定的伽马射线诱导的缺陷。
DOI：
10.1093/genetics/144.4.1735
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Westerfield,M
中科院分区：
文献类型：
--
作者：
Fritz,A;Rozowski,M;Walker,C;Westerfield,M
在斑马鱼中容易产生突变使得这种脊椎动物成为发育遗传学和基因组研究的重要资源。我们已经开发了一种基于PCR的筛选方法，可以有效地识别γ射线诱导的缺陷，针对选定的序列。我们描述了三个突变体的特征，我们的研究结果表明，这些突变包括缺失和易位，可以影响多达1%的基因组。这些缺陷提供了一个基础，分析克隆斑马鱼基因的功能，使用非互补筛选点突变诱导的高效化学诱变。
The ease with which mutations can be generated in zebrafish makes this vertebrate an important resource for developmental genetics and genome studies. We have developed a PCR-based screening method that allows the efficient identification of gamma-ray induced deficiencies targeted to selected sequences. We describe three mutants characteristic of our findings and show that these mutations include deletions and translocations that can affect as much as 1% of the genome. These deficiencies provide a basis for analyzing the functions of cloned zebrafish genes using noncomplementation screens for point mutations induced by high-efficiency chemical mutagenesis.