Detection of Bacteria in Bladder Mucosa of Adult Females
Detection of Bacteria in Bladder Mucosa of Adult Females
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DOI：
10.1097/ju.0000000000003189
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发表时间：
2023-05-01
影响因子：
6.6
通讯作者：
Walker, Stephen J.
中科院分区：
文献类型：
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作者：
Wolfe, Alan J.;Rademacher, David J.;Walker, Stephen J.
目的：间质性膀胱炎/膀胱疼痛综合征是一种慢性泌尿系统疾病，在美国有近800万女性被诊断出。尿微生物群是否发挥病因作用仍然存在争议。大多数研究评估了间质性膀胱炎/膀胱疼痛综合征患者的微生物群，并将排尿或插管尿液作为膀胱尿路上皮的代表;然而，尿液可能并不是膀胱微生物群的真实反映。膀胱活检组织可以提供更准确、因此更具临床相关性的膀胱微生物群图片。材料和方法：膀胱活检组织来自：（1）30例女性间质性膀胱炎/膀胱疼痛综合征（18-80岁）在治疗性膀胱水扩张后通过膀胱镜引导的冷杯活检，和（2）10名接受盆腔器官脱垂修复术的非间质性膀胱炎/膀胱疼痛综合征女性。为了检测细菌，对每个RNAlater保存的活检组织的技术复制品进行16 S rRNA基因测序。为了可视化细菌，paraformalde-fixed，石蜡包埋的活检组织进行了联合多重荧光原位杂交和荧光免疫组织化学分析和共聚焦microscopic.Results：细菌检测16 S rRNA基因测序在至少1个技术重复的大多数活检。最丰富的属是葡萄球菌属，其次是乳酸杆菌属;大肠杆菌属常见但不丰富。间质性膀胱炎/膀胱疼痛综合征患者与对照组之间无显著差异（P > 0.05）。结合荧光原位杂交和免疫组化可重复检测16 S rRNA上皮细胞和脱落细胞中尿路上皮和病变区域和毛细血管壁固有层的人膀胱活检tissue.Conclusions：我们的结论是，尿路上皮和尿液微生物群是相似的，但不相同的成年女性。
Purpose: Interstitial cystitis/bladder pain syndrome is a chronic urological condition diagnosed in nearly 8 million females in the United States. Whether urinary microbiota play an etiological role remains controversial. Most studies assessed the microbiota of interstitial cystitis/bladder pain syndrome patients with voided or catheterized urine as a proxy for bladder urothelium; however, urine may not be a true reflection of the bladder microbiota. Bladder biopsy tissue may provide a more accurate, and thus more clinically relevant, picture of bladder microbiota.Materials and Methods: Bladder biopsy tissues were obtained from: (1) 30 females with interstitial cystitis/bladder pain syndrome (18-80 years old) via cystoscopically guided cold-cup biopsy following therapeutic bladder hydrodistention, and (2) 10 non-interstitial cystitis/bladder pain syndrome females undergoing pelvic organ prolapse repair. To detect bacteria, technical duplicates of each RNAlater-preserved biopsy were subjected to 16S rRNA gene sequencing. To visualize bacteria, paraformaldehyde-fixed, paraffin-embedded biopsies were subjected to a combined multiplexed fluorescence in situ hybridization and fluorescence immunohistochemistry assay and confocal microscopy.Results: Bacteria were detected by 16S rRNA gene sequencing in at least 1 technical duplicate of most biopsies. The most abundant genus was Staphylococcus, followed by Lactobacillus; Escherichia was common but not abundant. There was no significant difference between interstitial cystitis/bladder pain syndrome patients and controls (P > .05). Combined fluorescence in situ hybridization and immunohistochemistry reproducibly detected 16S rRNA in epithelial cells and shed cells in the urothelium and lesioned areas and capillary walls in the lamina propria of human bladder biopsy tissue.Conclusions: We conclude that urothelial and urinary microbiota are similar but not identical in adult females.