A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus
A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus
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DOI：
10.1128/aem.62.5.1747-1751.1996
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发表时间：
1996-05-01
影响因子：
4.4
通讯作者：
Brahamsha, B
中科院分区：
文献类型：
--
作者：
Brahamsha, B
聚球藻属单细胞蓝藻是公海微微浮游生物中最丰富的成员之一，它们对初级生产力的贡献是相当大的。虽然一些分离株已被用于其独特的海洋环境适应性的生理、生化和分子研究，但为了使对这些生物及其独特性质的研究取得进展，有必要开发一种或多种海洋蓝藻模型的分子遗传学方法。聚球藻WH7803、WH8102和WH8103菌株的遗传操作已经发展了许多分子工具，其中包括高效获得分离菌落的平板技术和同时引入复制载体和自杀载体的接合方法。此外，还提供了一种产生随机的、标记的染色体插入的方法(N.Dolganov和A.R.Grossman，J.Billiol，175：7644-7651,1993；N.F.Tsinorems，A.K.Kutach，C.A.Strayer和S.Golden，J.Billiol)。176：6764-6768,1993)已经应用于这些生物。
Unicellular cyanobacteria of the genus Synechococcus are among the most abundant members of the picoplankton in the open ocean, and their contribution to primary production is considerable. While several isolates have been used for physiological, biochemical, and molecular studies of their unique adaptations to the marine environment, it has become necessary to develop molecular genetic methods for one or more model open-ocean cyanobacteria in order for studies of these organisms and their unique properties to progress. A number of molecular tools for the genetic manipulation of Synechococcus sp, strains WH7803, WH8102, and WH8103 have been developed, These include a plating technique for obtaining isolated colonies at high efficiencies and a conjugation method for introducing both a replicative vector and a suicide vector. In addition, a method for the generation of random, tagged chromosomal insertions (N. Dolganov and A. R. Grossman, J. Bacteriol, 175:7644-7651, 1993; N. F. Tsinoremas, A. K. Kutach, C. A. Strayer, and S. S. Golden, J. Bacteriol. 176:6764-6768, 1993) has been applied to these organisms.