Neuropeptide F from endocrine cells in Plutella xylostella midgut modulates feeding and synergizes Cry1Ac action
Neuropeptide F from endocrine cells in Plutella xylostella midgut modulates feeding and synergizes Cry1Ac action
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小菜蛾中肠内分泌细胞的神经肽 F 调节摄食并协同 Cry1Ac 作用
DOI：
10.1002/arch.21845
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发表时间：
2021
影响因子：
2.2
通讯作者：
Liu Xianjin
中科院分区：
文献类型：
--
作者：
Zhu Qing;Wang Jingxuan;Gao Meijing;Lu Lina;Liu Xianjin
随着转基因植物在世界范围内的广泛种植和对苏云金芽孢杆菌晶体（Cry）毒素抗性风险的增加，设计一种可持续使用的适应性抗性管理策略至关重要。昆虫神经肽F（NPF）在控制昆虫取食行为方面具有重要作用，对新型转基因植物的培育具有重要价值。本研究从小菜蛾中克隆了编码NPF的基因。真实的实时定量逆转录聚合酶链反应和原位杂交显示P. x-npf（P. x-npf），并参与了P. xylostellafeeding behavior和Cry 1Ac诱导的摄食抑制。原核表达和纯化提供了未折叠的结构。x-npffrom包涵体用于饮食表面覆盖生物测定，结果表明P. x-npfon Cry 1Ac毒性通过增加含有Cry毒素的有毒食物的摄入，特别是在喂养的早期阶段快速死亡。我们的研究结果提供了一种潜在的新方法，通过增加低剂量Cry毒素的摄入量来有效地控制害虫，并为复杂的Cry毒素机制提供了新的线索。
With the wide cultivation of transgenic plants throughout the world and the rising risk of resistance toBacillus thuringiensiscrystal (Cry) toxins, it is essential to design an adaptive resistance management strategy for continued use. Neuropeptide F (NPF) of insects has proven to be valuable for the production of novel‐type transgenic plants via its important role in the control of feeding behavior. In this study, the gene encoding NPF was cloned from the diamondback moth,Plutella xylostella, an important agricultural pest. Real‐time quantitative reverse transcription‐polymerase chain reaction and in situ hybridization showed a relatively high expression ofP. xylostella‐npf(P. x‐npf) in endocrine cells of the midgut of fourth instar larvae, and it was found to participate inP. xylostellafeeding behavior and Cry1Ac‐induced feeding inhibition. Prokaryotic expression and purification provided structure unfoldedP. x‐npffrom inclusion bodies for diet surface overlay bioassays and the results demonstrated a significant synergistic effect ofP. x‐npfon Cry1Ac toxicity by increasing intake of noxious food which contains Cry toxins, especially quick death at an early stage of feeding. Our findings provided a potential new way to efficiently control pests by increasing intake of lower dose Cry toxins and a novel hint for the complex Cry toxin mechanism.