The effects of chronic serum sickness on albumin distribution and glucose utilization in rat brain.
The effects of chronic serum sickness on albumin distribution and glucose utilization in rat brain.
复制标题
慢性血清病对大鼠脑白蛋白分布和葡萄糖利用的影响。
DOI：
10.1007/bf00305873
复制
发表时间：
1991
影响因子：
12.7
通讯作者：
Fenstermacher,J
中科院分区：
文献类型：
--
作者：
Nakata,H;Shimizu,A;Tajima,A;Lin,SZ;Gruber,K;Perillo,E;Peress,N;Fenstermacher,J
脑脊液（CSF）蛋白水平在与循环免疫复合物（如血清病）相关的疾病和疾病模型中升高。已知循环免疫复合物存款在有孔毛细血管的基底层中，因此可能影响毛细血管床和实质功能。由于脑内存在有孔和无孔毛细血管，慢性血清病时免疫复合物在有孔毛细血管内不同程度地沉积存款，某些脑区的脑毛细血管对蛋白质的通透性可能发生改变，导致CSF蛋白质升高。此外，各种其他脑血管和代谢功能也可能受到这种情况的影响。在本研究中，将放射性碘化血清白蛋白（RISA）或2-[14 C]脱氧葡萄糖（14 C-2DG）静脉注射到对照Wistar大鼠和慢性血清病Wistar大鼠中;随后通过定量放射自显影术测量了4个有窗孔毛细血管脑区和11个无窗孔毛细血管脑区的组织放射性水平。RISA的2分钟分布，划分灌注微血管中的循环血浆的体积，通常与局部血浆流量成比例，在对照组和实验组大鼠中是相同的。在两组中，RISA从血液进入大脑的时间超过30分钟，可忽略不计;因此，在慢性血清病引起的这15个脑区中，脑毛细血管对白蛋白的通透性均未检测到增加。根据14 C-2DG数据计算局部脑葡萄糖利用率（局部代谢和神经活动的指标），在对照组和实验组大鼠中几乎相同。这些结果表明，慢性血清病在这一阶段几乎没有影响毛细血管床的通透性和实质功能，在大多数，如果不是全部，脑区。
The level of cerebrospinal fluid (CSF) protein is elevated in diseases and disease models that are associated with circulating immune complexes such as serum sickness. Circulatory immune complexes are known to deposit in the basal lamina of fenestrated capillaries and may, as a result, affect both capillary bed and parenchymal function. Since the brain has both fenestrated and unfenestrated capillaries and immune complexes deposit to a varying extent in the fenestrated capillaries in chronic serum sickness, cerebral capillary permeability to protein may be altered in some brain areas and lead to the elevation of CSF proteins. In addition various other cerebrovascular and metabolic functions may also be affected by this condition. In this study either radio-iodinated serum albumin (RISA) or 2-[14C]deoxyglucose (14C-2DG) was intravenously injected into control Wistar rats and Wistar rats with chronic serum sickness; subsequently the tissue levels of radioactivity were measured by quantitative autoradiography in 4 brain areas with fenestrated capillaries and 11 brain areas with unfenestrated capillaries. The 2-min distribution of RISA, which demarcates the volume of circulating plasma in perfused microvessels and is generally proportional to local plasma flow, was the same in control and experimental rats. The passage of RISA from blood into brain over 30 min was negligible in both groups; thus cerebral capillary permeability to albumin was not detectably increased in any of these 15 brain areas by chronic serum sickness. The rate of local cerebral glucose utilization, an indicator of local metabolic and neural activity, was calculated from the14C-2DG data and was virtually identical in control and experimental rats. These results suggest that chronic serum sickness at this stage has little effect on capillary bed permeability and parenchymal function in most, if not all, brain areas.