ANTIBODY-FORMING-CELLS IN HUMAN COLOSTRUM AFTER ORAL IMMUNIZATION
ANTIBODY-FORMING-CELLS IN HUMAN COLOSTRUM AFTER ORAL IMMUNIZATION
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DOI：
10.1038/257797a0
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发表时间：
1975-01-01
期刊：
影响因子：
64.8
通讯作者：
SOHLAKERLUND, A
中科院分区：
文献类型：
--
作者：
GOLDBLUM, RM;AHLSTEDT, S;SOHLAKERLUND, A
有人建议用母乳喂养婴儿可以减少胃肠道感染的发生率。尽管乳汁中的巨噬细胞、淋巴细胞和抗体可能在这种保护中起重要作用2,3，但对肠道病原体产生特异性免疫的机制尚不清楚。人类初乳细胞可以合成IgA（参考文献4），我们已经使用溶血凝胶技术证明人类初乳含有许多细胞，这些细胞可以产生针对常见大肠杆菌O抗原的IgA抗体5。这表明致敏淋巴细胞要么在乳腺内经历了抗原诱导的克隆增殖，要么在致敏后从其他部位归巢到该器官。由于有报道称无菌小鼠的胃肠道免疫导致局部和全身产生IgA抗体，并且Peyer’s patches的细胞有效地在致命辐照兔的回肠中重新填充产生IgA的细胞，因此我们研究了胃肠道免疫对人类初乳中产生抗体细胞的影响。我们发现口服一种非致病性大肠杆菌菌株。产生对抗生物体O抗原的抗体的初侧细胞迅速出现。
IT has been suggested that feeding infants with human milk reduces the incidence of gastrointestinal infections1. Although macrophages, lymphocytes and antibodies in the milk are probably important in this protection2,3, the mechanism for the development of specific immunity to enteric pathogens is not known. Human colostral cells synthesise IgA (ref. 4), and we have used the haemolysis-in-gel technique to demonstrate that human colostrum contains numerous cells which produce IgA antibodies against the O antigens of commonly encounteredEscherichia colibacteria5. This suggested that the sensitised lymphocytes had either undergone antigen-induced clonal proliferation within the mammary gland or had homed to that organ from another site after becoming sensitised. Because of reports that gastrointestinal immunisation of germ-free mice led to local6and systemic7production of IgA antibodies, and that cells from Peyer's patches effectively repopulate the ileum of lethally-irradiated rabbits with IgA-producing cells8, we have examined the effect of gastrointestinal immunisation on the development of antibody-producing cells in colostrum of humans. We found that oral administration of a non-pathogenic strain ofE. coliled to the rapid appearance of colostral cells producing antibodies against the O antigen of the organism.