Serum and salivary antigliadin antibodies and serum IgA anti-endomysium antibodies as a screening test for coeliac disease
Serum and salivary antigliadin antibodies and serum IgA anti-endomysium antibodies as a screening test for coeliac disease
复制标题
DOI：
10.1111/j.1651-2227.1996.tb14157.x
复制
发表时间：
1996-07-01
期刊：
影响因子：
3.8
通讯作者：
Giera, B
中科院分区：
文献类型：
--
作者：
Rujner, J;Socha, J;Giera, B
通过酶联免疫吸附测定法测定了 18 名绒毛萎缩儿童和 30 名因乳糜泻而接受无麸质饮食但肠粘膜正常的儿童的血清和唾液 IgA 和 IgG 抗麦醇溶蛋白抗体。还通过免疫荧光法测定了这些儿童的血清 IgA 抗肌内膜抗体。血清 IgG 抗麦胶蛋白和 IgA 抗肌内膜抗体的敏感性最高（分别为 100 和 94.4%），其次是血清 IgA 抗麦胶蛋白抗体（72.2%）、唾液 IgA 抗麦胶蛋白抗体（61.2%）和 IgG 抗麦胶蛋白抗体（50%）。血清 IgA 抗肌内膜抗体 (100%) 和 IgA 抗麦胶蛋白抗体 (96.6%) 以及唾液 IgA 和 IgG 抗麦胶蛋白抗体 (93.3%) 的特异性最高，而血清 IgA 抗麦胶蛋白抗体的特异性最低 (63.3%)。
Serum and salivary IgA and IgG antigliadin antibodies were determined by an enzyme-linked immunosorbent assay in 18 children with villous atrophy and 30 children on a gluten-free diet for coeliac disease in whom normal intestinal mucosa was found. Serum IgA anti-endomysium antibodies were also determined by an immunofluorescence method in these children. Serum IgG antigliadin and IgA anti-endomysium antibodies had the highest sensitivity (100 and 94.4%, respectively), followed by serum IgA antibodies to gliadin (72.2%), salivary IgA antigliadin (61.2%) and IgG antigliadin (50%) antibodies. The highest specificity was found for serum IgA anti-endomysium (100%) and IgA antigliadin (96.6%) antibodies and salivary IgA and IgG antigliadin antibodies (93.3%), while serum IgA antigliadin antibodies were found to be least specific (63.3%).