Mouse thyroid primary culture
Mouse thyroid primary culture
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DOI：
10.1006/bbrc.1999.0468
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发表时间：
1999-04-13
影响因子：
3.1
通讯作者：
Caturegli, P
中科院分区：
文献类型：
--
作者：
Jeker, LT;Hejazi, M;Caturegli, P
技术进步大大减少了分析基因表达和编码蛋白质功能所需的细胞数量，使得即使是像小鼠甲状腺这样的小器官也可以进行体外研究。我们已经建立了小鼠甲状腺的原代培养物，在接种后长达14天，甲状腺球蛋白的细胞质染色很强。然后染色逐渐减少，并且在第28天仅存在于5 - 10%的甲状腺细胞中。此外，培养的甲状腺细胞表达的甲状腺过氧化物酶和促甲状腺素受体基因，虽然在较低的水平，钠碘转运体基因。最后，培养的甲状腺细胞可以通过脂质体转染瞬时转染，使用FuGENE 6。因此，我们报告，它是可能的培养功能的原代小鼠甲状腺细胞，可用于各种生物学研究。这个系统是有吸引力的，因为它允许使用的转基因，敲除和敲入小鼠品系在研究甲状腺病理生理学的数量不断增加。(C)北京：科学出版社.
Technological advances have drastically decreased the number of cells required to analyze expression of the genes and functions of the encoded proteins, making even a small organ like a mouse thyroid amenable to study in vitro. We have established primary cultures of mouse thyroids that showed, for up to 14 days after seeding, strong cytoplasmic staining for thyroglobulin. The staining then gradually decreased and was present in only 5-10% of thyrocytes at day 28. Furthermore, cultured thyrocytes expressed the thyroperoxidase and thyrotropin-receptor genes, and, although at lower levels, the sodium-iodide symporter gene. Finally, cultured thyrocytes could be transiently transfected by lipofection, using FuGENE 6. Thus, we report that it is possible to cultivate functional primary mouse thyrocytes that can be used for a variety of biological studies. This system is appealing because it permits the use of the ever-increasing number of transgenic, knock-out and knock-in mouse strains in studying thyroid pathophysiology. (C) 1999 Academic Press.