Instantaneous and continuous measurement of 14C-labeled substrate oxidation to 14CO2 by minute tissue specimens: an ionization chamber method.
Instantaneous and continuous measurement of 14C-labeled substrate oxidation to 14CO2 by minute tissue specimens: an ionization chamber method.
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通过微小组织样本瞬时连续测量 14C 标记底物氧化成 14CO2：电离室方法。
DOI：
10.1016/0026-0495(81)90138-4
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Soll,AH
中科院分区：
文献类型：
--
作者：
Davidson,WD;Klein,KL;Kurokawa,K;Soll,AH
振簧静电计和电离室已被用于瞬时和连续测量微量离体组织对14 C标记底物氧化为14 CO2的反应。这种改进的技术，利用“封闭”循环孵育系统，是10-50倍的灵敏度，如前所述的“开放”循环技术。底物氧化曲线描述了人类红细胞和多形核白细胞，犬壁细胞和大鼠肾单位的分离部分。该装置应该被证明是少量分离组织代谢研究的有用工具。
The vibrating reed electrometer and ionization chamber have been adapted for the instantaneous and continuous measurement of14C-labeled substrate oxidation to14CO2by minute quantities of isolated tissues. This modified technique, utilizing a “closed” circulation incubation system, is 10–50 times as sensitive as the previously described “open” circulation techniques. Substrate oxidation curves are described for human erythrocytes and polymorphonuclear leucocytes, canine parietal cells and isolated segments of the rat nephron. This apparatus should prove to be a useful tool for metabolic studies of small quantities of isolated tissue.