In vitro and in vivo antibiofilm activity of a coral associated actinomycete against drug resistant Staphylococcus aureus biofilms
In vitro and in vivo antibiofilm activity of a coral associated actinomycete against drug resistant Staphylococcus aureus biofilms
复制标题
DOI：
10.1080/08927014.2010.511200
复制
发表时间：
2010-01-01
期刊：
影响因子：
2.7
通讯作者：
Pandian, Shunmugiah Thevar Karutha
中科院分区：
文献类型：
--
作者：
Bakkiyaraj, Dhamodharan;Pandian, Shunmugiah Thevar Karutha
金黄色葡萄球菌是目前造成血流医院感染和留置医疗器械上生物膜形成的最重要的病原菌之一。其对常见抗生素的耐药性不断增加，部分归因于其形成生物膜的能力，这对开发新的抗微生物剂是一个挑战。因此，本研究的目的是评估珊瑚伴生放线菌（CAA）- 3对S。金黄色葡萄球菌生物膜的体外和体内研究。CAA-3的甲醇提取物显示出减少S.金黄色葡萄球菌ATCC 11632、耐甲氧西林的S.金黄色葡萄球菌ATCC 33591和临床分离的S.金黄色葡萄球菌的生物膜抑制浓度（BIC）为0.1 mg ml（-1）。此外，共聚焦激光扫描显微镜（CLSM）研究提供了CAA-3抑制肠道定殖的证据。金黄色葡萄球菌在线虫秀丽隐杆线虫中。总之，本研究首次报道了CAA作为抗生物膜化合物的有前途的来源，用于开发针对高度耐药葡萄球菌生物膜的新药。
Staphylococcus aureus is now amongst the most important pathogenic bacteria responsible for bloodstream nosocomial infections and for biofilm formation on indwelling medical devices. Its increasing resistance to common antibiotics, partly attributed to its ability to form biofilms, is a challenge for the development of new antimicrobial agents. Accordingly, the goal of this study was to evaluate the effect of a coral associated actinomycete (CAA) - 3 on S. aureus biofilms both in vitro and in vivo. Methanolic extracts of CAA-3 showed a reduction in in vitro biofilm formation by S. aureus ATCC 11632, methicillin resistant S. aureus ATCC 33591 and clinical isolates of S. aureus at the biofilm inhibitory concentration (BIC) of 0.1 mg ml(-1). Furthermore, confocal laser scanning microscope (CLSM) studies provide evidence of CAA-3 inhibiting intestinal colonisation of S. aureus in the nematode Caenorhabditis elegans. To conclude, this study for the first time, reports CAA as a promising source of anti-biofilm compounds, for developing novel drugs against highly resistant staphylococcal biofilms.