The human mammary gland basement membrane is integral to the polarity of luminal epithelial cells
The human mammary gland basement membrane is integral to the polarity of luminal epithelial cells
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DOI：
10.1006/excr.1998.4340
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发表时间：
1999-02-25
影响因子：
3.7
通讯作者：
Coombes, RC
中科院分区：
文献类型：
--
作者：
Slade, MJ;Coope, RC;Coombes, RC
我们发现肌上皮细胞基底膜衍生的层粘连蛋白-1的E3和E8结构域能够在上皮膜抗原定位方面使腔上皮细胞极化。这一事件依赖于α 6整合素，并导致黏附激酶复合物酪氨酸残基的聚集和磷酸化。我们还证明，与恶性上皮细胞一样，未培养的正常腔上皮细胞合成正常水平的β和γ层粘连蛋白链，并降低α链mRNA的水平。相反，正常的肌上皮细胞合成层粘连蛋白-1的所有三个组成链。因此，在乳腺癌中，肌上皮细胞的缺失可能导致层粘连蛋白α链的缺失，这可能导致恶性上皮细胞极性的丧失。(C) 1999学术出版社。
We show that myoepithelial cell basement membrane derived E3 and E8 domains of laminin-1 are capable of polarizing luminal epithelial cells with regard to epithelial membrane antigen localization. This event is dependent on the alpha 6 integrin and results in aggregation and phosphorylation of the tyrosine residues of the focal adhesion kinase complex. We also demonstrate that uncultured normal luminal epithelial cells synthesize normal levels of beta and gamma laminin chains and reduced levels of alpha chains mRNA in common with malignant epithelial cells. In contrast normal myoepithelial cells synthesize all three constituent chains of laminin-1. Therefore in breast cancer the absence of myoepithelial cells could result in a lack of laminin alpha chains which may contribute to loss of polarity of malignant epithelial cells. (C) 1999 Academic Press.