Quantitative changes in protein expression of cadmium-exposed poplar plants
Quantitative changes in protein expression of cadmium-exposed poplar plants
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DOI：
10.1002/pmic.200701110
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发表时间：
2008-06-01
期刊：
影响因子：
3.4
通讯作者：
Renaut, Jenny
中科院分区：
文献类型：
--
作者：
Kieffer, Pol;Dommes, Jacques;Renaut, Jenny
镉 (Cd) 污染是世界范围内的一个主要问题，它对植物产生有害影响。在目前的工作中，评估了 14 天水培培养中 20 μM Cd 暴露对杨树幼叶的影响。通过 2-D DIGE 分析蛋白质，然后进行 MALDI-TOF-TOF 鉴定。此外，在实验过程中监测生长和其他生理参数。经处理的植物表现出生长抑制，7天后出现视觉症状。 ICP-MS 分析记录了所有器官中 Cd 的显着积累。鉴定出具有各种功能的蛋白质表达的许多变化；特别是氧化应激调节蛋白的丰度下降，而发病机制相关蛋白的丰度却急剧增加。此外，大量参与碳代谢的蛋白质丰度下降，而参与从其他能源中重新利用碳的蛋白质则上调。总之，镉的负面影响可以通过对初级碳代谢和氧化应激反应机制的蛋白质表达的有害影响来解释。杨树茎中镉的积累，加上镉对生理参数影响较小，促进了杨树用于植物修复的用途。
Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 mu M Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification. Additionally, growth and other physiological parameters were monitored during the experiment. Treated plants exhibited an inhibition of growth and visual symptoms appeared after 7 days. A significant accumulation of Cd in all organs was recorded by ICP-MS analysis. A number of changes in the expression of proteins with various functions were identified; in particular a decreased abundance of oxidative stress regulating proteins, whereas pathogenesis-related proteins showed a drastic increase in abundance. Furthermore, a large number of proteins involved in carbon metabolism showed a decrease in abundance, while proteins involved in remobilizing carbon from other energy sources were upregulated. In conclusion, the negative effect of Cd could be explained by a deleterious effect on protein expression from the primary carbon metabolism and from the oxidative stress response mechanism. Accumulation of Cd in stems of poplar, coupled with a low impact of Cd on physiological parameters, promotes the use of poplar trees for phytoremediation purposes.