STRUCTURE OF THE GOLGI-APPARATUS IN STIMULATED AND NONSTIMULATED ACINAR-CELLS OF MAMMARY-GLANDS OF THE RAT
STRUCTURE OF THE GOLGI-APPARATUS IN STIMULATED AND NONSTIMULATED ACINAR-CELLS OF MAMMARY-GLANDS OF THE RAT
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DOI：
10.1002/ar.1092370303
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发表时间：
1993-11-01
影响因子：
--
通讯作者：
HERMO, L
中科院分区：
文献类型：
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作者：
CLERMONT, Y;XIA, L;HERMO, L
用电镜观察了3组大鼠乳腺腺泡细胞高尔基体的结构特征：（1）产后8天泌乳期的雌性大鼠（对照组），（2）与8天龄仔鼠分离后2 ~ 30小时处死的雌性大鼠（对照组）;和（3）雌性与其8日龄幼仔分离12小时，并返回其窝仔1、2、4和8小时。在组2的动物中，高尔基体堆叠在2和8小时之间保持与对照组相同。在12小时及以后，高尔基体堆栈的大小逐渐减少，但组成堆栈的元素的数量保持类似的哺乳期女性和所有含有酪蛋白亚胶束。在24和30小时，典型的分泌颗粒含有酪蛋白胶束消失的反方面的堆栈。在第2组大鼠的高尔基体中观察到的最早和最显著的变化发生在12小时。此时，前分泌颗粒和分泌颗粒的体积明显减少，并失去了大部分的电子透明内容物。这表明小分子的递送，即，乳糖和H2O，这些结构很快被改变后逮捕的吸吮刺激。在第3组动物中，前分泌颗粒和分泌颗粒的大小及其电子可透过的内容物的量在4小时恢复正常。因此，乳糖和H2O流入这些结构似乎在将幼崽送回其母亲后迅速恢复。在泌乳停止后12、18和24小时（第2组），高尔基体堆积的大小减少，伴随着在高尔基体堆积旁边和“孔”中形成簇的小泡数量增加。因此，在这些退化的高尔基体中，许多相关的小泡似乎是由囊泡的囊泡形成而产生的。 (C)1993 Wiley-Liss，Inc.
The structural features of the Golgi apparatus of acinar cells of mammary glands were examined with the electron microscope in 3 groups of rats: (1) in lactating female animals at 8 days postpartum, which served as controls; (2) in female rats sacrificed at various intervals from 2 to 30 hours following separation from their 8-day old pups; and (3) in females separated from their 8-day-old pups for a period of 12 hours and returned to their litters for durations of 1, 2, 4, and 8 hours. In animals of group 2, the Golgi stacks remained identical to that of controls between 2 and 8 hours. At 12 hours and later, the Golgi stacks decreased progressively in size, but the number of elements composing the stacks remained similar to that of lactating females and all contained casein submicelles. At 24 and 30 hours, typical secretory granules containing casein micelles disappeared from the trans aspect of the stacks. The earliest and most striking changes observed in the Golgi apparatus of the rats of group 2 took place at 12 hours. At this time, the prosecretory and secretory granules decreased considerably in volume and lost most of their electron-lucent content. This indicated that the delivery of small molecules, i.e., lactose and H2O, to these structures was soon altered following arrest of the sucking stimulus. In animals of group 3, the size of prosecretory and secretory granules and the amount of their electron-lucent content reverted to normal at 4 hours. Thus the influx of lactose and H2O into these structures appears to be rapidly restored after returning the pups to their mothers. The decrease in size of the Golgi stacks noted at 12, 18, and 24 hours following arrest of lactation (group 2), was accompanied by an increase in number of small vesicles that formed clusters next to the Golgi stacks and in ''wells.'' Thus in these regressing Golgi stacks, many of the associated small vesicles appear to arise by vesiculation of the saccules. (C) 1993 Wiley-Liss, Inc.