First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas
First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas
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DOI：
10.1016/j.lfs.2014.07.012
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发表时间：
2015-05-15
期刊：
影响因子：
6.1
通讯作者：
Uthus, Eric
中科院分区：
文献类型：
--
作者：
Anderson, Cindy M.;Ralph, Jody L.;Uthus, Eric
目的：由于母亲的维生素D状态已与先兆子痫，本研究的目的是确定DNA甲基化模式的变化和相关的蛋白质表达的胎盘基因调节维生素D metabolism.Main方法：一个方便的样本48怀孕未经产妇，包括11后来诊断为先兆子痫，被招募在这项前瞻性研究。在两组妇女中采用病例对照设计，我们进行了食物频率问卷调查，以确定维生素D的饮食摄入量。实验室测量包括血清维生素D水平（25[OH]D）、DNA甲基化模式和调节维生素D代谢的胎盘基因蛋白质表达（la-羟化酶，CYP 27 B1;维生素D受体，VDR;类维生素A X受体，RXR）从那些诊断为先兆子痫的人和那些在整个怀孕期间血压保持正常的人中分娩时收集的胎盘组织。维生素D饮食摄入量或平均血清25[OH]D水平无显著差异，尽管先兆子痫组25[OH]D水平缺乏的妇女比例（46%）高于血压正常组（20%）。先兆子痫妇女的胎盘样本也增加了CYP 27 B1，VDR和RXR基因的DNA甲基化，蛋白质表达水平较低，仅限于RXR.Significance：参与维生素D代谢的关键胎盘基因的超甲基化表明，可能干扰胎盘形成和维生素Oat母胎界面的可用性的过程解偶联。(C)2014 Elsevier Inc. All rights reserved.
Aims: As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism.Main methods: A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (la-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy.Key findings: There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46%) than the normotensive group (20%). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR.Significance: Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin Oat the maternal-fetal interface. (C) 2014 Elsevier Inc. All rights reserved.