Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1).
Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1).
复制标题
A/WSN/33(H1N1) 流感 PA 基因的核苷酸序列。
DOI：
10.1093/nar/18.3.654
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发表时间：
1990
影响因子：
14.9
通讯作者：
K. Tobita
中科院分区：
文献类型：
--
作者：
T. Odagiri;K. Tobita
用适当的限制性内切核酸酶切割克隆的DNA，并将其亚克隆到M13噬菌体载体mpl 8和mpl 9（1）中。通过双脱氧核苷酸链终止法（2）使用改良的T7聚合酶测序酶测定cDNA两条链的序列。A/WSN的PA基因全长2233个核苷酸，编码716个氨基酸，与A/PR/8/34（HINI）的PA基因同源性高达97%（3）。
The cloned DNA was cut with the appropriate restriction endonucleases and was subcloned into the M13 phage vectors mpl8 and mpl9 (1). The sequence was determined on both strands of the cDNA by the dideoxynucleotide chain termination method (2) using modified T7 polymerase sequenase. The PA gene of A/WSN contained 2233 nucleotides which can code for 716 amino acids and indicated a high homology (97%) to that of A/PR/8/34 (HINI)(3).