Increased expression of YKL-40, a chitinase-like protein, in serum and lung of patients with idiopathic pulmonary fibrosis
Increased expression of YKL-40, a chitinase-like protein, in serum and lung of patients with idiopathic pulmonary fibrosis
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DOI：
10.1016/j.rmed.2010.02.026
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发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Chida, Kingo
中科院分区：
文献类型：
--
作者：
Furuhashi, Kazuki;Suda, Takafumi;Chida, Kingo
背景资料：YKL-40是几丁质酶样蛋白的哺乳动物成员，已显示在导致组织重塑和纤维化的病理状况中发挥作用。最近，发现YKL-40在严重哮喘中增加，表明YKL-40有助于气道重塑;然而，没有关于YKL-40在特发性肺纤维化（IPF）中表达的数据。方法：采用酶联免疫吸附法（ELISA）检测63例IPF患者血清和18例IPF患者支气管肺泡灌洗液（BALF）中YKL-40的表达，并分析其临床意义。还评估了健康受试者的血清和BALF中的YKL-40水平。我们进一步研究了血清YKL-40水平与临床参数之间的关系。结果：IPF患者血清和BALF中YKL-40水平均显著高于正常对照组（血清：245.8 +/- 180.2 ng/ml vs. 116.0 +/- 58.3 ng/ml; BALF：17.8 +/- 19.1 ng/ml vs. 0.3 +/- 0.9 ng/ml）。血清YKL-40水平与血清KL-6水平和AaDO（2）呈显著正相关，与DLco和PaO 2呈负相关。免疫组化研究显示，增强YKL-40的表达在肺泡巨噬细胞和支气管上皮细胞相邻的纤维化病变在IPF，但不是在controls.Conclusions：这些数据表明，YKL-40是增加在循环和肺的IPF患者，这表明，这种糖蛋白与IPF的病理生理。(C)2010爱思唯尔有限公司版权所有。
Background: YKL-40, a mammalian member of chitinase-like proteins, has been shown to play a role in pathological conditions leading to tissue remodeling and fibrosis. Recently, YKL-40 was found to be increased in severe asthma, suggesting that YKL-40 contributes to airway remodeling; however, no data are available about YKL-40 expression in idiopathic pulmonary fibrosis (IPF). The present study was conducted to investigate YKL-40 expression in the serum and lung of IPF patients, and to determine its clinical significance.Methods: Using an enzyme-linked immunosorbent assay, we measured YKL-40 levels in the serum of 63 IPF patients and in bronchoalveolar lavage fluid (BALF) of 18 IPF patients. YKL-40 levels were also assessed in the serum and BALF of healthy subjects. We further investigated the relationship between serum YKL-40 levels and clinical parameters. Additionally, immunohistochemical staining for YKL-40 was performed in lung specimens of IPF patients and control subjects.Results: Serum and BALF YKL-40 levels were significantly higher in IPF than in controls (serum: 245.8 +/- 180.2 ng/ml vs. 116.0 +/- 58.3 ng/ml; BALF: 17.8 +/- 19.1 ng/ml vs. 0.3 +/- 0.9 ng/ml, respectively). Serum YKL-40 levels significantly correlated positively with serum KL-6 levels and AaDO(2), and negatively with DLco and PaO2. Immunohistochemical study revealed enhanced YKL-40 expression in alveolar macrophages and bronchiolar epithelia adjacent to fibrotic lesions in IPF, but not in controls.Conclusions: These data suggest that YKL-40 is increased in the circulation and lungs of IPF patients, suggesting that this glycoprotein is associated with the pathophysiology of IPF. (C) 2010 Elsevier Ltd. All rights reserved.