Performance of Kiestra total laboratory automation combined with MS in clinical microbiology practice.
Performance of Kiestra total laboratory automation combined with MS in clinical microbiology practice.
复制标题
DOI：
10.3343/alm.2014.34.2.111
复制
发表时间：
2014-03
影响因子：
4.9
通讯作者：
Visser CE
中科院分区：
文献类型：
--
作者：
Mutters NT;Hodiamont CJ;de Jong MD;Overmeijer HP;van den Boogaard M;Visser CE
微生物实验室寻求技术创新的解决办法，以科普大量样本和有限的人力和财力资源。最近变得可用的一个平台是Kiestra Total Laboratory Automation（TLA）系统（BD Kiestra B. V.，荷兰）。这套全自动样品处理系统配备了数字成像技术，可对微生物生长进行上级检测。将该方法与基质辅助激光解吸电离飞行时间质谱法（MS）（Bruker Daltonik，德国）相结合，预期能够更快速地鉴定病原体。通过使用Kiestra TLA结合MS的数字成像进行的早期生长检测与常规检测方法（CM）进行了比较。在219株临床血培养分离株中评价了两种方法的准确性和微生物鉴定所需时间。根据抗生素治疗处方评估早期微生物鉴定可能的临床影响。与CM相比，使用Kiestra TLA与MS组合的病原体鉴定导致每个分离株的时间增加30.6小时。在所有测试的分离物中，98.4%（249/253）的病原体被成功鉴定。在12%（24/200）的患者中，未进行药敏试验的早期微生物鉴定导致了抗生素方案的调整。与使用CM相比，Kiestra TLA与MS联合使用将缩短微生物病原体达到足够生长以进行药敏试验和鉴定所需的24小时孵育时间。这种方法不仅可以优化工作流程并降低成本，而且可以更快地启动抗生素方案中可能挽救生命的转换。
Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One platform that has recently become available is the Kiestra Total Laboratory Automation (TLA) system (BD Kiestra B.V., the Netherlands). This fully automated sample processing system, equipped with digital imaging technology, allows superior detection of microbial growth. Combining this approach with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) (Bruker Daltonik, Germany) is expected to enable more rapid identification of pathogens. Early growth detection by digital imaging using Kiestra TLA combined with MS was compared to conventional methods (CM) of detection. Accuracy and time taken for microbial identification were evaluated for the two methods in 219 clinical blood culture isolates. The possible clinical impact of earlier microbial identification was assessed according to antibiotic treatment prescription. Pathogen identification using Kiestra TLA combined with MS resulted in a 30.6 hr time gain per isolate compared to CM. Pathogens were successfully identified in 98.4% (249/253) of all tested isolates. Early microbial identification without susceptibility testing led to an adjustment of antibiotic regimen in 12% (24/200) of patients. The requisite 24 hr incubation time for microbial pathogens to reach sufficient growth for susceptibility testing and identification would be shortened by the implementation of Kiestra TLA in combination with MS, compared to the use of CM. Not only can this method optimize workflow and reduce costs, but it can allow potentially life-saving switches in antibiotic regimen to be initiated sooner.
登录
查看更多内容
影响因子：
9.4
作者：
van Veen, S. Q.;Claas, E. C. J.;Kuijper, Ed J.
通讯作者：
Kuijper, Ed J.
DOI：
10.1016/0021-9681(79)90012-2
发表时间：
1979-01-01
期刊：
JOURNAL OF CHRONIC DISEASES
影响因子：
--
作者：
SACKETT, DL
通讯作者：
SACKETT, DL
影响因子：
11.8
作者：
Seng, Piseth;Drancourt, Michel;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
4.9
作者：
Kang, CI;Kim, SH;Choe, KW
通讯作者：
Choe, KW
影响因子：
3.7
作者：
La Scola B;Raoult D
通讯作者：
Raoult D