Prevalence and genetic diversities of hantaviruses in rodents in Beijing, China
Prevalence and genetic diversities of hantaviruses in rodents in Beijing, China
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DOI：
10.4269/ajtmh.2008.78.98
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Cao, Wu-Chun
中科院分区：
文献类型：
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作者：
Jiang, Jia-Fu;Zuo, Shu-Qing;Cao, Wu-Chun
对在中国北京捕获的835只啮齿动物进行了汉坦病毒感染检测。55例（6.6%）肺组织样本进行了逆转录-聚合酶链反应检测病毒RNA阳性。在采集的666份血清中，ELISA检测IgG抗体阳性50份，阳性率为7.5%。在50只血清阳性啮齿动物中，37只为病毒RNA阳性。此外，5只啮齿动物病毒RNA阳性，但IgG抗体阴性。不同调查点（chi（2）= 28.93，df = 8，P = 0.001）和不同生境（chi（2）= 22.88，df = 7，P = 0.001）的感染率差异无统计学意义。P = 0.02）有显著性差异。11种代表性啮齿动物汉坦病毒M片段部分序列的变异率为0.1-8.2%。系统发育分析表明，我们的汉坦病毒序列属于三个不同的谱系，无论地理来源或啮齿动物物种。从某农产品交易中心检出的一株可能是从外省进口的菌株，与北京市的其他菌株在遗传上存在差异。
A total of 835 rodents captured in Beijing, China, were tested for hantavirus infection. Fifty-five (6.6%) were positive for viral RNA when lung tissue samples were examined by reverse transcriptase-polymerase chain reaction. Of 666 sera collected from the above rodents, 50 (7.5%) were positive for IgG antibody by ELISA. Among the 50 seropositive rodents, 37 were positive for viral RNA. In addition, five rodents were positive for viral RNA but negative for IgG antibody. The infection rates among study sites (chi(2) = 28.93, df = 8, P = 0.001) and habitats (chi(2) = 22.88,df = 7. P = 0.02) were significantly different. The sequences of partial M-segment of hantaviruses detected in 11 representative rodents had 0.1-8.2% divergence. Phylogenetic analysis showed that our hantavirus sequences fell into three different lineages regardless of geographical origin or rodent species. A strain detected from a trading center of agricultural products, which might be imported from other provinces, was genetically different from other strains of Beijing.