Proteomic profile of the saliva and plasma protein layer adsorbed on Ti-Zr alloy: the effect of sandblasted and acid-etched surface treatment
Proteomic profile of the saliva and plasma protein layer adsorbed on Ti-Zr alloy: the effect of sandblasted and acid-etched surface treatment
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DOI：
10.1080/08927014.2020.1769613
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发表时间：
2020-05-26
期刊：
影响因子：
2.7
通讯作者：
Barao, Valentim Adelino Ricardo
中科院分区：
文献类型：
--
作者：
Souza, Joao Gabriel Silva;Bertolini, Martinna;Barao, Valentim Adelino Ricardo
钛-锆合金是一种广泛应用于种植设备的生物材料，通常采用喷砂-酸蚀(SLA)的方法来改善其生物响应。虽然蛋白质吸附是第一个生物反应，但这种SLA处理对从唾液和血浆中吸附的蛋白质的蛋白质组谱的影响尚未得到测试。本研究采用LC-MS/MS联用技术对蛋白质组学图谱进行了分析，并以血链球菌为研究对象，考察了蛋白质层对细菌黏附的影响。SLA处理影响了蛋白质组图谱，显示从唾液(14)和血浆(3)吸附的蛋白质是唯一的。然而，这两组人在共同蛋白质、分子功能和由蛋白质介导的生物过程中表现出相似的强度模式。有趣的是，钛-锆(SLA)对血浆蛋白涂层的表面表现出更高的细菌粘附性(接近1.9倍)。因此，钛锆合金的SLA处理改变了蛋白质组图谱，这可能影响细菌的粘附性。
Titanium-zirconium (Ti-Zr) alloy has been widely used as a biomaterial for implant devices, and it is commonly treated by sandblasting followed by acid etching (SLA) to improve biological responses. Although protein adsorption is the first biological response, the effect of this SLA treatment on the proteomic profile of proteins adsorbed from saliva and blood plasma has not been tested. In this study, the proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).Streptococcus sanguiniswas used to test whether the protein layer affects bacterial adhesion. SLA treatment affected the proteomic profile, showing exclusive proteins adsorbed from saliva (14) and plasma (3). However, both groups exhibited close patterns of intensity for common proteins, molecular functions and biological processes mediated by proteins. Interestingly, Ti-Zr(SLA)showed higher bacterial adhesion (similar to 1.9 fold over) for the surface coated with plasma proteins. Therefore, SLA treatment of Ti-Zr alloy changed the proteomic profile, which may affect bacterial adhesion.