Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma.
Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma.
复制标题
DOI：
--
复制
发表时间：
2011-04
影响因子：
2
通讯作者：
D. Uejima;K. Nishijo;Y. Kajita;T. Ishibe;T. Aoyama;S. Kageyama;H. Iwaki;Takashi Nakamura;H. Iida;T. Yoshiki;J. Toguchida
中科院分区：
文献类型：
--
作者：
D. Uejima;K. Nishijo;Y. Kajita;T. Ishibe;T. Aoyama;S. Kageyama;H. Iwaki;Takashi Nakamura;H. Iida;T. Yoshiki;J. Toguchida
编码γ-谷氨酰环化转移酶的基因C7 orf 24的表达上调是来源于各种组织的癌症中的常见事件，但其在骨肉瘤（OS）中的参与尚未得到证实。材料和方法分析C7 orf 24在人OS细胞系和原发性肿瘤样品中的表达。使用针对C7 orf 24的siRNA研究了C7 orf 24对OS细胞系中的生长、运动和侵袭的生物学效应。通过全基因组基因表达谱分析寻找与C7 orf 24功能相关的基因。结果C7 orf 24在骨肉瘤细胞系和骨肉瘤中的表达水平明显高于正常成骨细胞。下调C7 orf 24表达抑制了与细胞聚集增强相关的细胞系的生长。用C7 orf 24-siRNA处理抑制细胞运动和侵袭。基因本体论认为C7 orf 24的功能与细胞粘附和蛋白质转运有关。结论C7 orf 24也参与了OS的生长，是一种潜在的肿瘤生物标志物。
BACKGROUND Up-regulation of the expression of the gene C7orf24, encoding γ-glutamyl cyclotransferase, is a common event in cancers derived from various tissues, but its involvement in osteosarcomas (OS) has not yet been demonstrated. MATERIALS AND METHODS The expression of C7orf24 was analyzed in human OS cell lines and primary tumor samples. The biological effects of C7orf24 on growth, motility, and invasion in the OS cell lines were investigated using siRNA for C7orf24. Genes related to the function of C7orf24 were sought by genome-wide gene expression profiling. RESULTS The level of C7orf24 expression was much higher in the OS cell lines and OS primary tumors than in normal osteoblasts. Down-regulation of C7orf24 expression inhibited the growth of the cell lines in association with enhancement of cell-clustering. Treatment with C7orf24-siRNA inhibited cell motility and invasion. Gene ontology suggested the function of C7orf24 to be related to cell adhesion and protein transport. CONCLUSION C7orf24 is also involved in the growth of OS, and is a potential biomarker for this type of tumor.