Analysis of intercellular washing fluids of potato tubers and detection of increased proteolytic activity upon fungal infection
Analysis of intercellular washing fluids of potato tubers and detection of increased proteolytic activity upon fungal infection
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马铃薯块茎细胞间冲洗液的分析以及真菌感染后蛋白水解活性增加的检测
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
C. Casalongué
中科院分区：
文献类型：
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作者：
F. Olivieri;A. Godoy;A. Escande;C. Casalongué
我们提出了提取马铃薯（Solamum tuberosum L. cv. Spunta）块茎细胞间液的第一个程序。从健康和镰刀菌感染的马铃薯组织中分离出细胞间洗涤液。使用 SDS-PAGE 的电泳图谱显示了两种组织液体之间的差异。在Fusarium solani f感染过程中积累了显着的细胞外蛋白水解活性。 sp。尤玛特。通过酪蛋白凝胶电泳分离细胞间液后，检测到表观分子量为 70 kDa 的主要蛋白水解带和另一个约 30 kDa 的蛋白水解带。蛋白水解活性主要受到氟磷酸二异丙酯的抑制，这表明丝氨酸蛋白酶的参与。体外降解试验表明，来自健康块茎的特定马铃薯蛋白质被来自受感染块茎的流体蛋白酶水解。这种活性在马铃薯-镰刀菌相互作用中的生物学作用仍不清楚。我们的结果表明细胞间丝氨酸蛋白酶具有真菌起源。
We present the first procedure for extracting intercellular fluids of potato (Solamum tuberosum L. cv. Spunta) tubers. Intercellular washing fluids were isolated from healthy and Fusarium-infected potato tissue. The electrophoretic pattern using SDS-PAGE indicated differences between the fluids from the two tissues. A significant extracellular proteolytic activity was accumulated during the infection with Fusarium solani f. sp. eumartii. A major proteolytic band with an apparent molecular mass of 70 kDa and another of approximately 30 kDa were detected after separation of intercellular fluids by casein gel electrophoresis. Proteolytic activity was principally inhibited by diisopropylfluorophosphate, which is indicative of the involvement of serine protease(s). In vitro degradation assay indicated that specific potato proteins from healthy tubers were hydrolyzed by fluid proteases from infected tubers. The biological role of such activity in potato-Fusarium interaction is still unknown. Our results suggest that the intercellular serine protease has a fungal origin.