Cell Surfactomes of Two Endometrial Epithelial Cell Lines That Differ in Their Adhesiveness to Embryonic Cells
Cell Surfactomes of Two Endometrial Epithelial Cell Lines That Differ in Their Adhesiveness to Embryonic Cells
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DOI：
10.1002/mrd.22301
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发表时间：
2014-04-01
影响因子：
2.5
通讯作者：
Sachdeva, Geetanjali
中科院分区：
文献类型：
--
作者：
Bhagwat, Sonali R.;Redij, Tejashree;Sachdeva, Geetanjali
子宫内膜上皮对胚胎的粘附性在妊娠的开始中起着关键作用。子宫内膜上皮细胞的丢失或获得也决定了子宫内膜上皮细胞转移的可能性，这是由某些遗传损伤引起的。采用基于蛋白质组学的对这些上皮细胞的探索，其可以鉴定可以破坏胚胎-子宫内膜相互作用和/或子宫内膜癌细胞转移的靶点。本研究确定了两个人子宫内膜上皮细胞系的表面分子，这两个细胞系因其与胚胎细胞的差异而闻名。RL 95 -2（表现出对胚胎细胞系JAr的更高的亲和力）和HEC-1A（表现出对JAr细胞的降低的亲和力）的表面组的比较性二维电泳分析揭示了55种差异富集的蛋白质。其中，通过MALDI-TOF/TOF或LC-MS/MS鉴定了10种蛋白质。TUBB 2C、ADAMTS 3和延伸因子β在HEC-1A细胞表面上更丰富，而HSP 27、HSPA 9、GP 96、CRT、Tapasin-ERP 57、PDI和β-肌动蛋白在RL 95 -2细胞表面上更丰富。同时采用Nano LC-MS/MS对RL 95 -2和HEC-1A的表面活性物质进行了表征。该研究还证明了CRT和HSPA 9的促粘附作用以及在细胞表面上发现的TUBB 2C群体的抗粘附作用。总之，本研究鉴定了两种人子宫内膜上皮细胞系的细胞表面蛋白互补物，并揭示了三种蛋白在异型细胞粘附中的作用。摩尔Reprod. Dev. 81：326-340，2014年。(c)2014 Wiley Periodicals，Inc.
Adhesiveness of the endometrial epithelium to an embryo plays a critical role in the initiation of pregnancy. Loss or gain of adhesiveness also dictates the potential of endometrial epithelial cells to metastasize, an event that can result from certain genetic insults. A proteomics-based exploration of the adhesiveness these epithelial cells was employed that could identify targets that could disrupt embryo-endometrium interactions and/or metastasis of endometrial cancer cells. The present study defined the surfactomes of two human endometrial epithelial cell lines known for their differential adhesiveness to embryonic cells. Comparative, two-dimensional electrophoretic analysis of the surfactomes of RL95-2 (exhibiting higher adhesiveness to the embryonic cell line JAr) and HEC-1A (exhibiting reduced adhesiveness to JAr cells) revealed 55 differentially enriched proteins. Of these, 10 proteins were identified by MALDI-TOF/TOF or LC-MS/MS. TUBB2C, ADAMTS3, and elongation factor beta were more abundant on the HEC-1A cell surface whereas HSP27, HSPA9, GP96, CRT, Tapasin-ERP57, PDI, and -actin were more abundant on the RL95-2 cell surface. Nano LC-MS/MS was also employed to generate a more comprehensive surfactomes of RL95-2 and HEC-1A. The study also demonstrated a pro-adhesive role of CRT and HSPA9 and an anti-adhesive role of TUBB2C populations found on the cell surface. In brief, this study identifies the cell-surface protein complements of two human endometrial epithelial cell lines, and reveals the role of three proteins in heterotypic cell adhesion. Mol. Reprod. Dev. 81: 326-340, 2014. (c) 2014 Wiley Periodicals, Inc.