Characterization of the short neuropeptide F receptor from Drosophila melanogaster
Characterization of the short neuropeptide F receptor from Drosophila melanogaster
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DOI：
10.1016/s0006-291x(02)02351-3
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发表时间：
2002-10-11
影响因子：
3.1
通讯作者：
Schoofs, L
中科院分区：
文献类型：
--
作者：
Mertens, I;Meeusen, T;Schoofs, L
从黑腹果蝇中克隆了一个七跨膜g蛋白偶联受体。该受体与脊椎动物神经肽Y-2受体结构相似，可被果蝇内源性肽激活，最近被称为短神经肽f （sNPFs）。迄今为止，在其他四种昆虫和马蹄蟹的神经内分泌组织中发现了单核苷酸多态性。在蝗虫中，它们加速卵巢成熟，在蚊子中，它们抑制寻找宿主的行为。RT-PCR表达分析表明，sNPF受体（Drm-sNPF-R）存在于果蝇幼虫的大脑、肠道、马尔比氏小管和脂肪体等多个组织中，也存在于成年雌性果蝇的卵巢中。所有4个果蝇sNPFs在表达受体的哺乳动物中国仓鼠卵巢细胞中明显引起钙反应。这种反应是剂量依赖性的，而且似乎非常特异性。短的NPF受体没有被任何其他测试的节肢动物肽激活，甚至没有被fmrfamide相关肽激活（也以RFamide结尾），这表明修饰的C端第4位的Arg残基似乎对sNPFs引起的反应至关重要(C) 2002 Elsevier Science （USA）。版权所有。
A seven transmembrane G-protein coupled receptor has been cloned from Drosophila melanogaster. This receptor shows structural similarities to vertebrate Neuropeptide Y-2 receptors and is activated by endogenous Drosophila peptides, recently designated as short neuropeptide Fs (sNPFs). sNPFs have so far been found in neuroendocrine tissues of four other insect species and of the horseshoe crab. In locusts, they accelerate ovarian maturation, and in mosquitoes, they inhibit host-seeking behavior. Expression analysis by RT-PCR shows that the sNPF receptor (Drm-sNPF-R) is present in several tissues (brain, gut, Malpighian tubules and fat body) from Drosophila larvae as well as in ovaries of adult females. All 4 Drosophila sNPFs clearly elicited a calcium response in receptor expressing mammalian Chinese hamster ovary cells. The response is dose-dependent and appeared to be very,specific. The short NPF receptor was not activated by any of the other tested arthropod peptides, not even by FMRFamide-related peptides (also ending in RFamide), indicating that the Arg residue at position 4 from the amidated C-terminus appears to be crucial for the response elicited by the sNPFs (C) 2002 Elsevier Science (USA). All rights reserved.