Association of plasma lipoproteins with postheparin lipase activities.
Association of plasma lipoproteins with postheparin lipase activities.
复制标题
血浆脂蛋白与肝素后脂肪酶活性的关联。
DOI：
10.1172/jci112744
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ginsberg,HN
中科院分区：
文献类型：
--
作者：
Goldberg,IJ;Kandel,JJ;Blum,CB;Ginsberg,HN
研究旨在探讨脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（HTGL）活性与人肝素后血浆（PHP）脂蛋白的关系。PHP凝胶过滤后的LPL活性的主要峰在富甘油三酯脂蛋白洗脱后和低密度脂蛋白（LDL）胆固醇峰值之前。当PHP含有乳糜微粒时，在柱的空隙体积中洗脱了一个额外的LPL活性峰。大部分HTGL活性在LDL洗脱后，在高密度脂蛋白胆固醇洗脱之前被洗脱。肝素前血浆中LPL活性相对于脂蛋白处于相同位置，与PHP中的LPL活性相同。纯化的人乳LPL与血浆或分离的低密度脂蛋白混合凝胶过滤，在低密度脂蛋白之前产生活性洗脱峰。在高盐缓冲液（1 M NaCl）中凝胶过滤PHP或在高盐密度溶液中超离心分离脂蛋白后，大多数脂肪酶活性与脂蛋白无关。通过含有载脂蛋白（apo） B和载脂蛋白e抗体的免疫亲和柱洗脱，从PHP中去除LPL活性。由于PHP中的脂蛋白在体内已经经历了事先的脂肪分解，因此PHP中的LPL活性可能与乳糜微粒和极低密度脂蛋白的残留物结合。
Studies were designed to explore the association of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) activities with lipoproteins in human postheparin plasma (PHP). The major peak of LPL activity after gel filtration of PHP eluted after the triglyceride-rich lipoproteins and just before the peak of low density lipoprotein (LDL) cholesterol. When PHP contained chylomicrons, an additional peak of LPL activity eluted in the void volume of the column. Most HTGL activity eluted after the LDL and preceded the elution of high density lipoprotein cholesterol. LPL activity in preheparin plasma eluted in the same position, relative to lipoproteins, as did LPL in PHP. Gel filtration of purified human milk LPL mixed with plasma or isolated LDL produced a peak of activity eluting before LDL. During gel filtration of PHP in high salt buffer (1 M NaCl) or after isolation of lipoproteins by ultracentrifugation in high salt density solutions, most of the lipase activity was not associated with lipoproteins. LPL activity was removed from PHP by elution through immunoaffinity columns containing antibodies to apolipoprotein (apo) B and apo E. Since lipoproteins in PHP have undergone prior in vivo lipolysis, LPL activity in PHP may be bound to remnants of chylomicrons and very low density lipoproteins.