Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells
Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells
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DOI：
10.1111/j.1365-2443.2009.01342.x
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发表时间：
2009-10-01
期刊：
影响因子：
2.1
通讯作者：
Nakano, Toru
中科院分区：
文献类型：
--
作者：
Kojima, Kanako;Kuramochi-Miyagawa, Satomi;Nakano, Toru
小鼠Piwi家族蛋白（MILI， MIWI和MIWI2）通过转录和转录后基因调控在精子发生中发挥关键作用。为了揭示这些蛋白的分子功能，我们研究了成年小鼠睾丸中与MILI结合的蛋白。我们发现MILI和MIWI都与TDRD1/MTR-1结合，这也是精子发生所必需的蛋白质。共免疫沉淀法和蛋白质及其突变体的亚细胞定位揭示了涉及MILI， MIWI和TDRD1/MTR-1的复合物形成。此外，在miwi缺失的圆形精子中，MILI和TDRD1/MTR-1的亚细胞定位发生改变，染色质体形成受损。这些数据表明，MILI， MIWI和TDRD1/MTR-1之间复合物的形成对于这些蛋白的整合亚细胞定位至关重要，并且可能是精子发生所必需的。
The mouse Piwi family proteins (MILI, MIWI and MIWI2) play pivotal roles in spermatogenesis through transcriptional and post-transcriptional gene regulation. To reveal the molecular functions of these proteins, we investigate the proteins that bind to MILI in adult mouse testes. We found that both MILI and MIWI bind to TDRD1/MTR-1, which is also an essential protein for spermatogenesis. Co-immunoprecipitation assays and subcellular localization of the proteins and mutants thereof revealed a complex formation involving MILI, MIWI and TDRD1/MTR-1. In addition, the subcellular localizations of MILI and TDRD1/MTR-1 were altered, and chromatoid body formation was impaired in the MIWI-null round spermatids. These data suggest that the formation of complexes between MILI, MIWI and TDRD1/MTR-1 is critical for the integrated subcellular localizations of these proteins, and is presumably essential for spermatogenesis.